Protein Info for QEN71_RS27795 in Paraburkholderia sabiae LMG 24235

Annotation: Do family serine endopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details amino acids 24 to 27 (4 residues), see Phobius details transmembrane" amino acids 22 to 23 (2 residues), see Phobius details TIGR02037: peptidase Do" amino acids 64 to 390 (327 residues), 434.2 bits, see alignment E=2.8e-134 PF13365: Trypsin_2" amino acids 118 to 252 (135 residues), 122.4 bits, see alignment E=6.4e-39 PF00089: Trypsin" amino acids 118 to 277 (160 residues), 71.1 bits, see alignment E=3.2e-23 PF00595: PDZ" amino acids 289 to 370 (82 residues), 48.5 bits, see alignment E=2.3e-16 PF13180: PDZ_2" amino acids 293 to 380 (88 residues), 59.1 bits, see alignment E=1.1e-19 PF17820: PDZ_6" amino acids 317 to 357 (41 residues), 47.2 bits, see alignment 3.9e-16

Best Hits

KEGG orthology group: K08070, 2-alkenal reductase [EC: 1.3.1.74] (inferred from 98% identity to bph:Bphy_2745)

Predicted SEED Role

"Outer membrane stress sensor protease DegQ, serine protease" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.74

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (402 amino acids)

>QEN71_RS27795 Do family serine endopeptidase (Paraburkholderia sabiae LMG 24235)
MLRRFWLFFAQAVTVLLALMFIIATLKPQWLQRQGQFGKQLAEPIVALREVAPGMGSGGQ
VQNSYADAAQKAMPAVVNVFSSKDGSLPPDPRAKDPLFRYFFGDKNKKQQEQPAANLGSG
VIVSSEGYILTNQHVVDGADQIEIALADGRTTNAKVIGVDPETDLAVLKVNMTNLPTITL
GRMDQTRVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTDAPINPGNSGG
ALVDVNGNLLGINTAIYSRSGGSLGIGFAIPVSTARSVLESIITTGSVTRGWIGVEPQDV
TPEIAESFGLEQKSGAIVAGVLKGGPADKAGIKPGDILVSVNGQDITDTTRLLNVIAQIK
PGTDAKVHLVRKNKEMDLTVMIGKRPPPPKQPSDEGDDEDGG