Protein Info for QEN71_RS27665 in Paraburkholderia sabiae LMG 24235

Annotation: glutamyl-tRNA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 transmembrane" amino acids 250 to 267 (18 residues), see Phobius details TIGR01035: glutamyl-tRNA reductase" amino acids 4 to 415 (412 residues), 419.4 bits, see alignment E=8e-130 PF05201: GlutR_N" amino acids 6 to 161 (156 residues), 182.4 bits, see alignment E=9.4e-58 PF01488: Shikimate_DH" amino acids 177 to 310 (134 residues), 155.5 bits, see alignment E=1.8e-49 PF00745: GlutR_dimer" amino acids 325 to 416 (92 residues), 92.2 bits, see alignment E=4.5e-30

Best Hits

Swiss-Prot: 98% identical to HEM1_PARP8: Glutamyl-tRNA reductase (hemA) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K02492, glutamyl-tRNA reductase [EC: 1.2.1.70] (inferred from 98% identity to bph:Bphy_2720)

MetaCyc: 46% identical to glutamyl-tRNA reductase (Escherichia coli K-12 substr. MG1655)
Glutamyl-tRNA reductase. [EC: 1.2.1.70]

Predicted SEED Role

"Glutamyl-tRNA reductase (EC 1.2.1.70)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (429 amino acids)

>QEN71_RS27665 glutamyl-tRNA reductase (Paraburkholderia sabiae LMG 24235)
MQLLTIGINHHTAPVALRERVAFPLEQIKPALSTFKDIFLGRTARTAPEAAILSTCNRTE
LYCATDDQAAREAAIHWLSKYHNIAIDELAPHVYALPQSEAVRHAFRVASGLDSMVLGET
QIVGQMKDAVRTATEAGALGTYLNQLFQRTFAVAKEVRTTTEIGAQSVSMAAAAVRLAQR
IFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVANRTAERGQRLAESFNGRAIPLSE
LPTRMHEFDIIVSCTASTLPIIGLGAVERAVKARRHRPIFMVDLAVPRDIEPEVGQLEDV
FLYTVDDLGAIVREGNASRQAAVAQAETIIETRVQNFMQWLDARSIVPVIRHMHTQADTL
RRAEVEKAQKMLARGDDPAAVLEALSQALTNKLIHGPTHALNRASSENRDQLIELMGGFY
KHAHSSSER