Protein Info for QEN71_RS27545 in Paraburkholderia sabiae LMG 24235

Annotation: phosphoglycolate phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 PF00702: Hydrolase" amino acids 17 to 198 (182 residues), 115.9 bits, see alignment E=6.4e-37 PF12710: HAD" amino acids 20 to 190 (171 residues), 34.7 bits, see alignment E=5.2e-12 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 21 to 225 (205 residues), 206.6 bits, see alignment E=6.8e-65 PF13419: HAD_2" amino acids 21 to 204 (184 residues), 132 bits, see alignment E=5.2e-42 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 87 to 202 (116 residues), 40.3 bits, see alignment E=7.2e-14 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 90 to 198 (109 residues), 33.8 bits, see alignment E=8.7e-12 PF13242: Hydrolase_like" amino acids 160 to 225 (66 residues), 40.2 bits, see alignment E=5.2e-14

Best Hits

Swiss-Prot: 41% identical to GPH_THIDA: Phosphoglycolate phosphatase (Tbd_2229) from Thiobacillus denitrificans (strain ATCC 25259)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 94% identity to bph:Bphy_2697)

Predicted SEED Role

"Phosphoglycolate phosphatase (EC 3.1.3.18)" in subsystem 2-phosphoglycolate salvage or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 3.1.3.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (242 amino acids)

>QEN71_RS27545 phosphoglycolate phosphatase (Paraburkholderia sabiae LMG 24235)
MESTPLFPAPAFTAGKIRAAIVDLDGTMVDTADDFTASLNGMLAQFDAEETTREEVIDYV
GKGSEHLIRSVLAPRFEADLAQSRFDEALAIYQEEYAKINGLHTRLYPDVEAGLIALREA
GLELACVTNKPHRFATQLLEHHGLLRYFSVVLGGDSVAAKKPDPLPMLTACERLGVSPQD
AVAIGDSQNDAFAGRAAGMSTLTVPYGYNHGEAVQTIKSDGIVVSLLGAAKAIAAHNAAN
KQ