Protein Info for QEN71_RS26540 in Paraburkholderia sabiae LMG 24235

Annotation: bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930 PF03710: GlnE" amino acids 12 to 254 (243 residues), 207.6 bits, see alignment E=3.3e-65 amino acids 552 to 791 (240 residues), 220.7 bits, see alignment E=3.2e-69 PF08335: GlnD_UR_UTase" amino acids 284 to 426 (143 residues), 110.5 bits, see alignment E=1.1e-35 amino acids 813 to 901 (89 residues), 30.3 bits, see alignment E=5.7e-11

Best Hits

Swiss-Prot: 79% identical to GLNE_BURMA: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Burkholderia mallei (strain ATCC 23344)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 98% identity to bph:Bphy_2506)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (930 amino acids)

>QEN71_RS26540 bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase (Paraburkholderia sabiae LMG 24235)
MTEPSLLSSSYSHYASRAYAAQPDLAARVAALTAAPITRERIDERLDALLNALRATPDAP
LSEDALKKALRQLRAEVFCAVMERDLSRAADVAEVTGTMTDLAEATVQRAMAVVSADLEA
LFGEPRGAQGERLSLGVVGMGKLGGRELNVSSDIDLIFVYEDDGETTGGQRSPIATQDFF
TRLGKRLIAALAEVTADGYVFRVDMRLRPNGDSGPLVCSLGMLEEYFYVQGREWERYAWI
KGRLVSEHASDSARRLSKQLDAIVKPFVYRRYLDFGVISAIRSLHLQIRQEAQRRASMRP
DKADDIKLGRGGIREIEFSAQVFQLIRGGQDAGFRVRPTLAVLRHAAARGLVSPGVCADL
TSAYLFLRDVEHRLQYRNDAQTHAMPVDPAERAHLAQAMGFDDYASLMAKLEAHRELVEQ
QFDQIFADKVSGQGGCGAREDGAAVWVWSSALTDDSADEALAARLVELGIERPADLLARL
KAVWQSSRYAGLAESSRQRFDIVAQRALEAAHTLEPAERRGDILVRLFDLLEAVGRRGAY
LALLTEYPQALHRVLSVLGASRWAAGYLIRHPQLLDELLDDEAMASPFDWPEFKRTLRAR
LAAADGVEHQMDLLRHAHQAEVFRILLIDLAGKLSVEHVSDRLSELADAVLDVTIEAVWN
QLAKRHRQVPRFAIIAYGKLGGKELGYASDLDLIFLYDDADDNASDIYATFTRRLITWLT
TATGAGTLFDVDLRLRPNGESGLLVTDLDAFRRYQLREGDAANTAWVWEHQALTRARYCA
GDPGIGAQFEAIREQVLTTPRDAATLSAEIVDMRRRVEEGHPNRTELFDLKHDRGGMVDI
EFAVQYWVLLHAARDPELIRNTGNIALLREVSRFGLMSGEEADTVGAAYRTYRKLQHKLR
LDGMEKARVEPQTVEKEREAVLRLWRRVFG