Protein Info for QEN71_RS26240 in Paraburkholderia sabiae LMG 24235

Annotation: bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 2 to 187 (186 residues), 165.7 bits, E=1.8e-52
• PF08659: KR
  • amino acids 3 to 163 (161 residues), 42.3 bits, E=1.6e-14
• PF13561: adh_short_C2
  • amino acids 7 to 221 (215 residues), 116.3 bits, E=3.5e-37

Best Hits

Swiss-Prot: 69% identical to YDFG_SALTI: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhi

KEGG orthology group: None (inferred from 95% identity to bph:Bphy_2450)

MetaCyc: 67% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

• superpathway of L-threonine metabolism (15/18 steps found)
• 3-hydroxypropanoate cycle (10/13 steps found)
• L-threonine degradation III (to methylglyoxal) (2/3 steps found)
• β-alanine degradation III (1/2 steps found)
• uracil degradation III (3/5 steps found)
• aminopropanol phosphate biosynthesis II (2/4 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• glyoxylate assimilation (8/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Protein Sequence (248 amino acids)

>QEN71_RS26240 bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG (Paraburkholderia sabiae LMG 24235)
MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQALADELGDNLLPFELDVRDRAA
VEAVPTALPADFAAVDVLVNNAGLALGLEPAQRAVLDDWTTMIETNCTGLVQVTRAFLPG
MIERNRGHVFNLGSVAASWPYPGGNVYGATKAFVRQFSLNLRADLHGTPIRVTDIEPGLC
GGTEFSNVRFRGDDEKAANVYQNVQPLTAEDIADSIYWIATRPAHVNINTIELMPVAQSF
AGLNIHRG