Protein Info for QEN71_RS24400 in Paraburkholderia sabiae LMG 24235

Annotation: phosphoribosylamine--glycine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 PF02844: GARS_N" amino acids 1 to 100 (100 residues), 132 bits, see alignment E=2.6e-42 TIGR00877: phosphoribosylamine--glycine ligase" amino acids 1 to 420 (420 residues), 566.3 bits, see alignment E=2e-174 PF01071: GARS_A" amino acids 101 to 294 (194 residues), 268 bits, see alignment E=1e-83 PF02786: CPSase_L_D2" amino acids 103 to 203 (101 residues), 20.5 bits, see alignment E=5.9e-08 PF02843: GARS_C" amino acids 329 to 418 (90 residues), 105.3 bits, see alignment E=3.4e-34

Best Hits

Swiss-Prot: 76% identical to PUR2_RALSO: Phosphoribosylamine--glycine ligase (purD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 98% identity to bph:Bphy_2065)

MetaCyc: 61% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (425 amino acids)

>QEN71_RS24400 phosphoribosylamine--glycine ligase (Paraburkholderia sabiae LMG 24235)
MKLLVVGSGGREHALAWKLAQSPRVQLVYVAPGNGGTAQDDRLRNIDITDPNALADFVEK
EQIAFTLVGPEQPLAAGIVNIFRARGMKIFGPSKEAAQLESSKDFAKAFMKRHAIPTAEY
ETFSDVAAAHAYIDAKGAPIVIKADGLAAGKGVVVALTLEEAHHAVDMMLSDNKLGDAGA
RVVIEEFLAGEEASFIVMVDGKNVLALASSQDHKRLLDGDQGPNTGGMGAYSPAPIVTPQ
LHARVMREIILPTVRGMEKEGIRFTGFLYAGLMIDAQGNPKTLEFNCRMGDPETQPIMAR
LKGDFSKVVEQAIAGTLDTVELEWDRRTALGVVLAAHNYPEAPRKGDFISGIPPETADAV
TFHAGTTLADGKLTTSGGRVLCVVGLADSVRSAQSVAYEAINHISFDGMQYRRDIGYRAA
NRKHG