Protein Info for QEN71_RS22800 in Paraburkholderia sabiae LMG 24235

Annotation: non-ribosomal peptide synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 26 to 374 (349 residues), 265.2 bits, E=4.5e-82
  • amino acids 2681 to 3020 (340 residues), 267.3 bits, E=1e-82
• TIGR01733: amino acid adenylation domain
  • amino acids 46 to 447 (402 residues), 388.2 bits, E=2.1e-120
  • amino acids 2701 to 3095 (395 residues), 398.2 bits, E=1.9e-123
• PF13193: AMP-binding_C
  • amino acids 431 to 505 (75 residues), 44.9 bits, see alignment (E = 9.4e-15)
  • amino acids 3079 to 3153 (75 residues), 30.9 bits, see alignment (E = 2.1e-10)
• PF00550: PP-binding
  • amino acids 536 to 597 (62 residues), 25.9 bits, see alignment (E = 4.9e-09)
  • amino acids 1545 to 1611 (67 residues), 36.3 bits, see alignment (E = 2.7e-12)
  • amino acids 3214 to 3274 (61 residues), 45.3 bits, see alignment (E = 4.3e-15)
• PF00109: ketoacyl-synt
  • amino acids 619 to 872 (254 residues), 242.2 bits, 3.5e-75
• PF00108: Thiolase_N
  • amino acids 735 to 821 (87 residues), 26.7 bits, see alignment (E = 1.8e-09)
• PF02801: Ketoacyl-synt_C
  • amino acids 880 to 999 (120 residues), 125.3 bits, see alignment (E = 6.7e-40)
• PF16197: KAsynt_C_assoc
  • amino acids 1002 to 1116 (115 residues), 59.1 bits, see alignment (E = 2.7e-19)
• PF00698: Acyl_transf_1
  • amino acids 1144 to 1435 (292 residues), 173.1 bits, E=5.4e-54
• PF00202: Aminotran_3
  • amino acids 1813 to 2133 (321 residues), 153.4 bits, E=3.9e-48
• PF00668: Condensation
  • amino acids 2222 to 2656 (435 residues), 281.8 bits, E=4.7e-87

Predicted SEED Role

"Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 2.3.1.39)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• fatty acid biosynthesis initiation (type II) (3/3 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
• fatty acid biosynthesis initiation (plant mitochondria) (1/4 steps found)
• mycobactin biosynthesis (3/11 steps found)
• pederin biosynthesis (2/14 steps found)
• bryostatin biosynthesis (2/19 steps found)
• mupirocin biosynthesis (2/26 steps found)
• corallopyronin A biosynthesis (2/30 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.39

Use Curated BLAST to search for 2.3.1.39

Protein Sequence (3306 amino acids)

>QEN71_RS22800 non-ribosomal peptide synthetase (Paraburkholderia sabiae LMG 24235)
MSHRDGFPGNLPGGTGHMPGVAARFDEVRAEFADHLAVIDSTGQESYAALGERSARLATV
LLAMGLQPGERCAIMVPRSRDTLALILAILRVGAVYVPLDPAYPRAQLDFIVADCEPRLI
IAEGSALASVGDLNGVLVDLARIVASSEAADPAPLHASHRDDPAYIMYTSGSTGKPKGVI
VPQRAILRLVHGQSFTALSPQTRFLNLAPLAFDASTLEIWGPLLNGGCAAIIDAVQPSLD
TIASDIARLGATSAWFTAGLFNALADYRLEAFRPLQEVLTGGDVLSPVHVRKVMDAHPSL
QIVNGYGPTENTTFSCCYRIPREGDALADGGAIPIGDAIAGTRVYIVDDKLVPVGEGEVG
ELVVGGDGVALGYLNRPELTAEKFVDDVFSPVAKLYRTGDLVRRRADGAIDFLGRNDRQI
KIAGKRIELDEIEHALRAAPGVADAAVAAFDAQTGKSIAAFVKSGAAPAVLQQQIRAHLK
SALPDYMMPTALRVLPDFPLTPNGKIDRKALLAGLDTTAATSAEAPPVDDDIAGKLASVF
EGLLGQAVDRRANFFDLGLRSLDLMRAHAIILRDVSAKVALVDLFRHPNVEALTTHLHAT
LETASDGSIRRRPDANSGAIAVVGMSGRFPGAHNVAGLWANILAGRDCITHFDVTELEDT
FDDSLRRDASYVKARPVLADVDRFDAGFFGVLAREAALTDPQQRLFLEIAWEAFEDAGYD
PATIAGAVGVFAGTSMNTYFLKHVLTDRGVIDEFTSQFQIGEYQKLVGAGDFVATRTAYK
LGLTGPAISVQTACSTSLTAIGMAVENLRAGRCDMALAGGVSITFPQKRGYFYQEGGMGA
PDGVCRPFDADAKGTVFGSGAGVVLLKRLDDAIADADPIYAVIRGVGINNDGSDKVGFTA
PSVDAQARAIAIAHAEAGIDPASVGYIETHGTATPLGDPIEFAGLVQAFRLGGVEGGQFC
ALGSAKANVGHLDAAAGVTGFIAAALALRDATLPPLLHFGSPNPGIDVSNSPFFFNVAAR
PWADGPTPRRAGVSSFGVGGTNVHVLLEEAPPREVAGESQRGVQILPLSARSAAALDRAK
ANLAQHLTTRPDTSLADVAATLQAGRRAFAHRAVVVADSVEQAQAKLQKGAIEGQAPQDA
LPVVFMFPGQGAQYPGMGDALYRTEPVYRQWIDKGAEALTPHLGLDIRTLLFSETPEGDD
TPHPIRSTIYAQPALFLVEYALAQLWISRGVKPAAMIGHSVGELVAACVAEAIAFEDALY
LIAQRGALMQSAEPGAMLVVRLPEAELKAILPADVDLAAINAPSLSVVAGPFAAIEAFES
TLKAGEIDHRRLHTSHAFHSRMMSGVVEDLAKLADTLSFAIPKIPYVSSVTGQWASMDQP
VTGRYWAGHCRDVVRFSDALGTVTSESRPVLLEIGPGRTLTTFASQGLPKDRHAGAIASL
PDFAMRERELAVLAEATGRVWLNGVTPDWKTVRADAARRVSLPTYPFERERYWIDAPATT
SSSRPQTTPAAAPAAPASPETTTDTPQTAMAQPAKIDRQPRLIAELASLLAEMSGEAPDT
SNPDVTFWDLGYDSLFMGQVSRQLRRRYDVTISFRQIMSDYPTLPALAQFLDGTLPPDPE
QPAVPAAAEAAPVAAAAPAAAVAAVAAAPIVVAAAPVMAAQFAPSAATGDIQSVIRDQIA
AMQSLMTRQLDVLQGAPVAIAQPAAAVQAAVAPAQIAQIVATAPVAVAAAPARAAGPEIK
FEENRPTRFTAYKPGATNSAQMSDAQGAFIRDLTSRYSAKTPKSKSRTQSYRSVLADPRT
ASGFREEWKELVYPIVAQRSKGSKIWDIDGNEYIDIVNGYGQTAFGHTPDFVVDAVNAQM
AEGFAIGPQSPLAGEVAQMFADMTGHQRVTFCNTGSEAVMAAMRLARTVTGKDKIVCFDG
DYHGQFDEVLVKPGSEAGVPRAFPLAPGIPQQSVGNMVVLPYARTESLEWIKANIDDIAG
VLFEAVQSRHPNLRPKEFVQQLREVTAANESALIFDEVVTGFRVHPAGMQGYWGIQGDMA
TYGKVVGGGLPVGVLAGSAQYMDALDGGQWQFGDNSVPEVPPTFFAGTFVRHPVVLAAMK
AVLLHLKEAGPALQDKLASRMDGLVQRINAYLEKVGIATRAESFSSWFYVSFAGEDRLGS
LFYPYMRYLGVHVMEGFPCFLTTSHSDEDIRKIGDAFIASIDALQRVGILGNAPLTAPAQ
LTQAPLTEPQKEIWMSAQVSHEASLAFNESFTLELNGELNEAAFERAFAATVARHDALRV
HFSRVGDMMFVDPATIVPLEKIDLSSHADAAEQLKTIIDAEAREVFDLTRGPLARAGLVR
LESQKWAFVFTAHHIVCDGWSINIILRDLSALYAAEVSGAQAELDEVQSFLAFAKAQDEA
GVDADTRDFWMNLHRNPAPQPDLPGDRPRPELKSFSGASTTRHLGADLLKQVKTASSKQG
CSLFATLFGAAQVLFGRLSNNDDVVIAAPMAGQSQAGEALLVGHCVNFLPLRVKFDRNQP
FATHMKAVRDHLYDAGDRQNYTYGALVRDLGVKRDLNRLPLTDLQFNLEKVDGELDMAGV
TTRFAPNAKACSNFDLFLNVIESAKGLRLDCDFNTDVYDEATIQRWLGYYENVLTAIAHD
AETTVAALSLLNEAEIHHLRDELNDSQRAYDLSHTTPAMIAAQARQTPNAPAVSDEVLRL
SYGELDASASQIARSLVAAGIAPRGRVAIAMDRSVMTVAAMIGVWRAGCAYVPLDMTMPP
ARLRQILDGADIAAILTDGASRAVLEPGAHRVLDLETLLAPRDDAAVTLPNVSETDPAYV
IFTSGSTGQPKGVEIPHRALSNFLLSMAEAPGFTAKDRIVAVTTFSFDISGLELFLPLIA
GGQTFIAGHAEVRTGYELVTRLNEQAATVLQATPSLWRMLLEAGFKAPQGFRILCGGEPL
PRDLADALLATGAEVWNLYGPTETTIWSSASRVVADGAVVIGAPLANTQLHVLTDDLHLA
PQGVSGELWIGGDGLAKGYFNRPDLTDAAFRSVSIEGAAPCRLYRTGDLAKRLADGSLQH
LGRRDQQIKLRGFRIEIEEIEAALRKAPGVAAAAVALHTVGDSPRLVGYIVSSAADKADQ
GAIAAHVAGQLPAYMVPTLWMTLDALPQTSNGKLDRKALPVPTADMVATPVRQPHAALKV
VPPAPISTGPAAADAITIEPVAAEPVALTQTQSTIAAIWGDVLGLQHVGIDQQFFSLGAD
SLQLFRIVARMNERGLGVDARQLMKNLTIAELAASLDGPPAEELMVAPAVARPSILNFKR
RVAEGV