Protein Info for QEN71_RS22690 in Paraburkholderia sabiae LMG 24235

Annotation: FMNH2-dependent alkanesulfonate monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 PF00296: Bac_luciferase" amino acids 1 to 330 (330 residues), 296.3 bits, see alignment E=1.5e-92 TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 2 to 354 (353 residues), 626.9 bits, see alignment E=4.3e-193

Best Hits

Swiss-Prot: 94% identical to SSUD_PARP8: Alkanesulfonate monooxygenase (ssuD) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 94% identity to bph:Bphy_1649)

MetaCyc: 66% identical to alkanesulfonate monooxygenase subunit (Pseudomonas putida DS1)
RXN-18086 [EC: 1.14.14.34]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.5

Use Curated BLAST to search for 1.14.14.34 or 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (385 amino acids)

>QEN71_RS22690 FMNH2-dependent alkanesulfonate monooxygenase (Paraburkholderia sabiae LMG 24235)
MNVFWFIPTHGDSRYLGTSQGARAADYDYFRQIAVAADTLGYEGVLLPTGRSCEDAWVVA
SSLIPATKRLKFLVAIRPGITSPGLSARMAATFDRLSGGRLLINVVTGGDAAELEGDGLF
VDHDTRYEITDEFLRIWRGQLAAAHHGESVDFIGRHLKSKGGKLLYPPVQNPHPPLWFGG
SSPAAHEMAGEHIDTYLTWGEPPEAVAKKIADIRARAAQHGRTIRFGIRLHVIVRETEDE
AWAAADKLISRLDDETVARAQASFSKMDSEGQRRMAALHGGKLGSRKDLEIYPNLWAGVG
LVRGGAGTALVGSPEQVAARMQEYAELGIETFILSGYPHLEESYRFAELVFPLLPGRQHA
SASGPLSGPFGEIVGNHYAPKASQS