Protein Info for QEN71_RS22370 in Paraburkholderia sabiae LMG 24235

Annotation: ATP-dependent chaperone ClpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 865 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 860 (855 residues), 1431.2 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 67 (51 residues), 49.2 bits, see alignment 3.7e-16 amino acids 94 to 145 (52 residues), 36.5 bits, see alignment 3.3e-12 PF00004: AAA" amino acids 203 to 319 (117 residues), 50.9 bits, see alignment E=1.7e-16 amino acids 608 to 728 (121 residues), 40.8 bits, see alignment E=2.3e-13 PF17871: AAA_lid_9" amino acids 342 to 442 (101 residues), 117.9 bits, see alignment E=1.3e-37 PF07724: AAA_2" amino acids 602 to 764 (163 residues), 232.8 bits, see alignment E=1.7e-72 PF00158: Sigma54_activat" amino acids 606 to 725 (120 residues), 24.6 bits, see alignment E=1.4e-08 PF07728: AAA_5" amino acids 607 to 728 (122 residues), 50.7 bits, see alignment E=1.5e-16 PF10431: ClpB_D2-small" amino acids 771 to 849 (79 residues), 86.5 bits, see alignment E=7.5e-28

Best Hits

Swiss-Prot: 79% identical to CLPB_RALSO: Chaperone protein ClpB (clpB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 92% identity to bcj:BCAL1919)

MetaCyc: 67% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (865 amino acids)

>QEN71_RS22370 ATP-dependent chaperone ClpB (Paraburkholderia sabiae LMG 24235)
MRIDKLTTKFQEALADAQSLAVGHDNQYIEPVHLLAALVAQQDGSARSLLARAGVHVQAL
QTALADAITRLPQVQGTDGNVQIGRDLTGLLNQADKEAQKLNDTYIASEMFLLSVADDKG
EAGRLAREHGLSRKSLEAAIVAVRGGAQVHSQDAESQREALKKYTIDLTERARAGKLDPV
IGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL
DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLK
PALSRGELHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPSVEATIAILRGLQERYELH
HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKMEIDSKPEEMDRLDRRRI
QLKIEREAVKKEKDEASQKRLQLIEEEIERLDREYSDLEEIWTAEKAAVQGSAQLKEEIE
KTRAEITRLQREGKLEKVAELQYGKLPQLEQQLKAVTKAEAEEQSNPTRPRLLRTQVGAE
EIAEVVSRATGIPVSRMMQGEREKLLQIEEKLHQRVVGQDEAISAVADAIRRSRAGLSDP
NRPYGSFLFLGPTGVGKTELCKALAAFLFDAEDHLIRIDMSEFMEKHSVARLIGAPPGYV
GYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
VMTSNLGSQVIQSMVGEPQDAVKDAVWEEVKLHFRPEFLNRIDDVVVFHALDRSNIQSIA
KIQLERVHERLAKLDMQLVVSDAALELIGKVGYDPLFGARPLKRAIQQEIENPVAKLILA
GKFGPKDVIPVEVDNGKLVFDRVVH