Protein Info for QEN71_RS20340 in Paraburkholderia sabiae LMG 24235

Annotation: ATP-dependent DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 2 to 554 (553 residues), 715 bits, see alignment E=3.5e-219 PF04675: DNA_ligase_A_N" amino acids 2 to 161 (160 residues), 43.3 bits, see alignment E=7.3e-15 PF01068: DNA_ligase_A_M" amino acids 234 to 426 (193 residues), 108.9 bits, see alignment E=4.4e-35 PF04679: DNA_ligase_A_C" amino acids 447 to 540 (94 residues), 41 bits, see alignment E=3.1e-14

Best Hits

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 94% identity to bph:Bphy_4680)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (560 amino acids)

>QEN71_RS20340 ATP-dependent DNA ligase (Paraburkholderia sabiae LMG 24235)
MKRFATLYTALDATTSTRDKLDALITYFHAAPPEDAAWASYFLAGGKPRQSVPTRLLTEI
ARTRAGLPEWLFEESYQAVGDLAETIAHVLPPAARTSELGLTQWIEERILTLRGASPETL
RERLIAYWDELDWSGRFLLTKLIGGGFRVGVARQLVVRALAEAAGVDHKLIAQRMVGWTD
SSQPPDAARYLRLIAPAPSADTQDDEAARIRHESELGLPYPFFLAHPLQADPSTLGDPSS
WFAEWKWDGIRAQVVKRDGRVWVWSRGEDLITDRFPEVVALGEALPDGYVLDGEILAWEP
NALAPLPFARLQPRITRKSLTKRVLAESPATFLAYDLLEANGDDLRMQPLQERRARLDTL
GAQLADTLAKDLLRVSPLVDASDWNALAALRDESRARGVEGLMLKERASMYGVGRTKAAG
TWWKWKIDPYAIDAVLIYAQRGHGRRASLYTDFTFAVWDEADGVRTLVPFAKAYSGLTDE
EMREVDAIVRKTTVEKFGPVRSVTPTLVFEIGFEGIQASPRHKSGVAVRFPRMLRWRTDK
HIDDADTLVTLKGFIDERAR