Protein Info for QEN71_RS19725 in Paraburkholderia sabiae LMG 24235

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 4 to 318 (315 residues), 383.1 bits, E=1.3e-118
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 7 to 761 (755 residues), 1108.6 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 154 to 333 (180 residues), 24.5 bits, E=1.6e-09
  • amino acids 435 to 757 (323 residues), 522.2 bits, E=5.6e-161

Best Hits

Swiss-Prot: 86% identical to METE_PARP8: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 86% identity to bph:Bphy_5470)

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Protein Sequence (763 amino acids)

>QEN71_RS19725 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (Paraburkholderia sabiae LMG 24235)
MVTTHNLGFPRIGAKRELKFALEKYWKNESSRDELKMVGAELRERHWKDQAALDFAPVGD
FSFYDQVLDMSFTLGNLPERVRGFHGDALDNYFRVARGRSARGAEDHSACCGGVAAGEMT
KWFDTNYHYIVPEFDAHTNFSLDPSRLLEQLTQARKLGVKTKPVIVGPVTYLWLGKAKDD
SDKLALLPRLLPVYAALLDYFTAQGIEWVQIDEPALVTELDATWRQAFRTAYEALSERRV
KLLLATYFGQLQDNLELACKLPVDGLHIDAINARDEVVAVVAQLPATSVVSVGAINGRNI
WKTDLTAALEWLEPLHRSIGDRLWIAPSCSLLHTPVDLSSEQKLDAEIKSWLAFALQKLD
ELTVLAGALNNGRESVAEALAANAKAIESRRTSPRVHNPAVKAAIARIDASLGKRASAYE
QRVKTQSAILNLPAFPTTTIGSFPQTNEIRHARSQFKAGELDQAGYKLAMEQEITRAVKE
QEALGLDVLVHGEAERNDMVEYFGEQLDGYVFSQFGWVQSYGSRCVKPPILFGDISRPKA
MTVEWISYAQSQTAKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIREEVLDLEKA
GVRVIQIDEAALREGLPLRRAQWNEYLQWAVESFRITANGVRDETQIHTHMCYSEFNDII
ASIADMDADVITIETSRSDMELLDAFDNFKYPNEIGPGVYDIHSPNIPSQEHIVNLMKKA
AERIPAGRLWVNPDCGLKTRQWAEVLPALTNMVAAAKTLRQSV