Protein Info for QEN71_RS17740 in Paraburkholderia sabiae LMG 24235

Annotation: DUF3416 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1147 transmembrane" amino acids 948 to 967 (20 residues), see Phobius details PF11896: GlgE_dom_N_S" amino acids 469 to 657 (189 residues), 184.5 bits, see alignment E=4.8e-58 PF00128: Alpha-amylase" amino acids 681 to 792 (112 residues), 35 bits, see alignment E=2.5e-12 PF21702: GLGE_C" amino acids 1026 to 1115 (90 residues), 63.3 bits, see alignment 4.1e-21

Best Hits

Swiss-Prot: 59% identical to GLGE_BURPS: Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (glgE) from Burkholderia pseudomallei (strain K96243)

KEGG orthology group: None (inferred from 95% identity to bph:Bphy_4393)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.1

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1147 amino acids)

>QEN71_RS17740 DUF3416 domain-containing protein (Paraburkholderia sabiae LMG 24235)
MEAPHAYAPRIYFVHSPLVGRLDAWPAQFEHAAALGFDHVLIGGLFQPGQAGHAQIVSDH
AKLHPALATDQPAHEVLQRLTEAASQHGLTLLVDLVIDRAAADGQLYREHRDWFHPFEPE
EARLDPRHARHEDNVAYADFSNEHVAAPLIDWWANTLDTLADAGIGGFRFDSPHRVPVFA
WRRLLDTVRASRHPSTRFLAATPGLMRSDIAALESVGFDAVFSSSRWWDFRAPWMLDEHA
VLMRAGSPIAFPEAPYGTRLAADLAQVHDSAIVERAYRRALLTAVSTGTGWLMPMGFEYG
IAEPISHTLGDAGHYANLRSAARFDLAERVRHANAIARDTWSLQTNGELRSLSGPDTHAA
LLLRGDEHDLRDAGEAVLIAINPELGTPVRIDPARFLDGVPGGFTRFVALGTEARSAPQP
LAAFTLPPGACRLFRAVAEKPVVLAPPIDKQNTKTSGHKSVLDAIAMSRVAIESVSPAVD
HGRFPAKRIVGERVHVTAAVFAEGHDKIAAAVIWRAADETAWHETPMTPAQPAGNDIWEA
RIPLERLGRHEFTVIAWRDDFASLVDHMQKKLKADQSVELEVEEAKHLFALALAEAETAE
GSQPQQLDAIVKEFMKADVGERLAIVLAPTTAEAFAAARHRPGLSRDQIVYRIDAERAGA
RFGSWYEIFPRSTSDDAHRHGNFDDVIGKLPRIRDMGFDVLYFPPIHPIGIANRKGRNNT
LTPGPDDVGSPYAIGGEAGGHDAVHPQLGTLDDFKRMLAAAHDHGLEIALDFAIQCSPDH
PWLKKHPTWFAWRPDGTLRYAENPPKKYQDIVNPDFYARDAKPELWISLRDVILFWIDAG
VRIFRVDNPHTKPFPFWEWMINDVRARHPDVIFLSEAFTRPRVMARLAKLGFSQSYTYFT
WRESKRDFTEYLTELTQTNLRDFFRPNFFVNTPDINPRHLQRQGRPGFLIRAALATMLSG
LWGVYSGFELCEAAALPNSEEYLDSEKYQIRAWDWNRPGNIVREITELNRIRRTNPALHT
HLNLTFLPAHNENILFFEKATEARDNVIVVAINLDPYNEQGADIELSWSTFNRWGVHDHG
AIDVIDQMTGEQFQWQGRWQHVRLDPGTRPFAIWRIAPAGGLPREAVPDEAEAHRAAHDT
TFNEGAI