Protein Info for QEN71_RS14030 in Paraburkholderia sabiae LMG 24235

Annotation: aromatic ring-hydroxylating dioxygenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 7 to 94 (88 residues), 71.8 bits, E=3.7e-24
• PF19112: VanA_C
  • amino acids 142 to 336 (195 residues), 165.2 bits, E=2.1e-52

Best Hits

KEGG orthology group: K03862, vanillate monooxygenase [EC: 1.14.13.82] (inferred from 67% identity to agr:AGROH133_08645)

Predicted SEED Role

"Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82)" in subsystem Phenylpropanoid compound degradation (EC 1.14.13.82)

MetaCyc Pathways

• vanillin and vanillate degradation II (2/2 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.82

Use Curated BLAST to search for 1.14.13.82

Protein Sequence (357 amino acids)

>QEN71_RS14030 aromatic ring-hydroxylating dioxygenase subunit alpha (Paraburkholderia sabiae LMG 24235)
MFLKNAWYVAAWDAEVTHALMSVTILGEPVVLYRREDGTPVALEDACPHRKLPLSMGRLI
GDHVECGYHGLTFDCEGACVRAPGSPRIPPGAKVRSYPLAERYGLLWIWMGNAEEADPST
IVQIGEWGDPAWGVNRGDVMTVACHYLYVTDNLLDPSHVAWVHPSSFGNAACEAEPLRTE
VAANGVTVSRWMRDVDVAPFYAQFVRFDGRCDRKQHYEVRFPSHAIIKAIFTPSGTGGDD
APPHADVFLMNSYNFMTPVDESNTRYYWFQTRNFAPDDEQVSRQFDADVRHAFEEDRVVL
TAVHAGMKNARTPNIDLAIDAGPLRFRRALAQMIEREQQASVATAPVYVVSRQRAEH