Protein Info for QEN71_RS12710 in Paraburkholderia sabiae LMG 24235

Annotation: M20 family metallopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01891: amidohydrolase
  • amino acids 21 to 370 (350 residues), 267.5 bits, E=9.6e-84
• PF01546: Peptidase_M20
  • amino acids 76 to 366 (291 residues), 105.1 bits, E=5.1e-34
• PF07687: M20_dimer
  • amino acids 200 to 290 (91 residues), 27.5 bits, E=2.6e-10

Best Hits

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 86% identity to bvi:Bcep1808_5855)

Predicted SEED Role

"Peptidase M20D, amidohydrolase"

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32

Protein Sequence (422 amino acids)

>QEN71_RS12710 M20 family metallopeptidase (Paraburkholderia sabiae LMG 24235)
MSKLVGTMASAIDILLPDLEAIYKDLHAHPELSMQEVRTAQIAADHAQKFGYEVTRNVGG
TGVVAVLRNGDGPTVMLRADMDALPMAEATGLPYASTVTARDEDGVEVGVAHSCGHDLHV
TWLMGVVRLMAEHRDAWRGTLMAVFQPGEEVGRGARSMIDDGMMTRFPKPDVILGQHVMV
GMAGTVSYRSGTILSAGDSLKVRLFGRGSHGSQPQTSVDPVIMAASTTLRLQTIVSREIS
PRDSAVLTIGALQAGTKENIIPDDATLKLNMRTFDDDVREYMLGAIRRICCAECAASNAP
REPEFTTLSSYPLTINDEATIERLGAAFKSHFGERAYEAQPAAASEDFSVFGREWGVPYA
FWFVGGTDPDQFKSALAAKKLNEIPSNHSPKFAPVLSPTLRTGLEAMLCAAGEWLGKLDG
DE