Protein Info for QEN71_RS12650 in Paraburkholderia sabiae LMG 24235

Annotation: SDR family NAD(P)-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 9 to 200 (192 residues), 198.6 bits, E=1.5e-62
• PF08659: KR
  • amino acids 11 to 162 (152 residues), 30.1 bits, E=9.1e-11
• PF01370: Epimerase
  • amino acids 11 to 190 (180 residues), 28.2 bits, E=2.4e-10
• PF13561: adh_short_C2
  • amino acids 16 to 246 (231 residues), 188.4 bits, E=3.2e-59

Best Hits

Swiss-Prot: 51% identical to KDRDH_SPHSS: 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)) (LRA5) from Sphingomonas sp. (strain SKA58)

KEGG orthology group: K00059, 3-oxoacyl-[acyl-carrier protein] reductase [EC: 1.1.1.100] (inferred from 96% identity to bph:Bphy_3470)

MetaCyc: 54% identical to 2-dehydro-3-deoxy-L-pentonate 4-dehydrogenase (Herbaspirillum huttiense)
RXN-12095 [EC: 1.1.1.401]; 1.1.1.401 [EC: 1.1.1.401]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.1.1.100)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• tetradecanoate biosynthesis (mitochondria) (23/25 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (12/12 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (17/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• biotin biosynthesis I (13/15 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• L-rhamnose degradation III (6/7 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• L-arabinose degradation V (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (25/205 steps found)
• superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100

Use Curated BLAST to search for 1.1.1.100 or 1.1.1.401

Protein Sequence (250 amino acids)

>QEN71_RS12650 SDR family NAD(P)-dependent oxidoreductase (Paraburkholderia sabiae LMG 24235)
MNRIDLEGRVVVVTGGARGIGYAVAQRALRSGASVALWDLDAGRLARSERELGELGKVAA
ITVDQTKEAQIDAAVSQTLAAFGAIDVLINCAGITGGNGTTWELDPDVWRQVIDVNLIGP
YLVCRAVVPQMLKQGYGRIVNIASVAGKEGNPNASHYSASKAGLIGLTKSLGKELATKNI
LVNAVTPAAAKTEIFDSMKQEHIDYMLSKIPMNRFLLPDEAASLILWLGSEDCAFSTGSV
FDLSGGRATY