Protein Info for QEN71_RS12445 in Paraburkholderia sabiae LMG 24235

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 PF01590: GAF" amino acids 62 to 191 (130 residues), 53.7 bits, see alignment E=1.2e-17 PF00158: Sigma54_activat" amino acids 327 to 489 (163 residues), 220.2 bits, see alignment E=5.1e-69 PF14532: Sigma54_activ_2" amino acids 330 to 495 (166 residues), 72.2 bits, see alignment E=1.9e-23 PF07728: AAA_5" amino acids 346 to 465 (120 residues), 26.1 bits, see alignment E=2.6e-09 PF02954: HTH_8" amino acids 602 to 639 (38 residues), 38.8 bits, see alignment 2.2e-13

Best Hits

KEGG orthology group: None (inferred from 92% identity to bph:Bphy_3427)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (645 amino acids)

>QEN71_RS12445 sigma-54-dependent Fis family transcriptional regulator (Paraburkholderia sabiae LMG 24235)
MTQRSVTPSVMGRADVLAQAHARSREFGLRASETPDFHPLQPFALRDLVERNRSLYAHAL
PVMETLHAQIVDTESMVLLTDSNGVILHSLGDTDFVEKASRVALCAGVSWSEADRGTNAV
GTALTEGQPTVVHGGEHYLEANRILTCSCVPIADPYGRTIGALDVSGDPRGFHKHTLALV
RMSAQIIENQLFANQFGNAVRVRFHARSEFIGTLFEGLAAFGGNGTLLAANRSALFQFGR
PLDTLLGQPFEALFGNAFPAVLQQASRAPGECLTLMLPSGVRVLARAEYAARPAFSADSG
MQRAPSATVSCKPRAAAITFATLDTGDARMAAVLGRVEKVRGRDIPLLVLGKTGTGKEWL
ARAMHEESPRRNAPFVAVNCASLPDTLIEAELFGYEDGAFTGARKRGNVGKIVQADGGTL
FLDEIGDMPLAQQVRLMRVLQERSVVPLGGTRAVPVDVRIVCATHRNLRAMIEEGTFRED
LYYRINGLVVTLPALAERTDLPVLVERILARLRNDEADSERMPVRVSADVLDCFERCRWP
GNLRQLANVLRTAGIMAEGEDEIGLEHLPDDFLHDCETPSDAPPLRALPGVDPHTPSRPA
NMEELQATLIEQTLARNNGNISAAARELGLARNTVYRYMRIRTTH