Protein Info for QEN71_RS11755 in Paraburkholderia sabiae LMG 24235

Annotation: type II asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00520: L-asparaginase, type II
  • amino acids 33 to 366 (334 residues), 431.2 bits, E=1.3e-133
• PF00710: Asparaginase
  • amino acids 45 to 236 (192 residues), 216.2 bits, E=3.1e-68
• PF17763: Asparaginase_C
  • amino acids 259 to 363 (105 residues), 97.4 bits, E=5.4e-32

Best Hits

Swiss-Prot: 59% identical to ASPG_DICCH: L-asparaginase (ansB) from Dickeya chrysanthemi

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 62% identity to srs:SerAS12_2462)

MetaCyc: 61% identical to N-(1-deoxy-D-fructos-1-yl)-L-asparaginase (Salmonella enterica enterica serovar Typhimurium str. LT2)
Asparaginase. [EC: 3.5.1.1]

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

• L-asparagine degradation I (1/1 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
• L-asparagine degradation III (mammalian) (2/3 steps found)
• N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Cyanoamino acid metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.1

Use Curated BLAST to search for 3.5.1.1

Protein Sequence (366 amino acids)

>QEN71_RS11755 type II asparaginase (Paraburkholderia sabiae LMG 24235)
MRDVHCLSRSYRRVVCKLLVIGCIFSEPTLAQTQPQSATQTPLPHVAVLATGGTIAGTAA
NETQSTGYKSGVLTADALLKSVPSLGKVAHVSGEQVSNVGSENITNEILLKLARRVNQLL
AQPDIAGVVITHGTDTLEETAYFLNLVVKSDKPVVLTGSMRPSTAISADGPSNILQAVTV
AASPTARGRGVMIVLNDRIGSARFTTKTNANTLDTFRSPDDGFLGTLIDNVPHFETTVAK
LQTTQTPFDVSKMDSLPEVDIVYGHQNDGGYMYDAAVAHGARGIVVAGVGAGSGSLLTLP
SIKRAIEKGVIVVRSSRTGSGYIPEDPAYVGLLGDNLNPQKARILLMLALTVTNNPERVQ
EIFHQF