Protein Info for QEN71_RS10470 in Paraburkholderia sabiae LMG 24235

Annotation: benzoate 1,2-dioxygenase electron transfer component BenC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00111: Fer2
  • amino acids 10 to 87 (78 residues), 66.1 bits, E=3.2e-22
• PF00970: FAD_binding_6
  • amino acids 111 to 203 (93 residues), 69.1 bits, E=5.2e-23
• PF00175: NAD_binding_1
  • amino acids 213 to 316 (104 residues), 77.6 bits, E=1.7e-25

Best Hits

Swiss-Prot: 67% identical to BENC_ACIAD: Benzoate 1,2-dioxygenase electron transfer component (benC) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K05784, benzoate 1,2-dioxygenase electron transfer component (inferred from 95% identity to bph:Bphy_5350)

MetaCyc: 54% identical to XylZ (Pseudomonas putida mt-2)
Benzoate 1,2-dioxygenase. [EC: 1.14.12.10]

Predicted SEED Role

"benzoate dioxygenase, ferredoxin reductase component" in subsystem Benzoate degradation

MetaCyc Pathways

• benzoate degradation I (aerobic) (2/2 steps found)
• mandelate degradation to acetyl-CoA (10/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (22/35 steps found)
• meta cleavage pathway of aromatic compounds (4/10 steps found)
• toluene degradation IV (aerobic) (via catechol) (4/13 steps found)
• superpathway of aerobic toluene degradation (12/30 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (16/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.10

Use Curated BLAST to search for 1.14.12.10

Protein Sequence (340 amino acids)

>QEN71_RS10470 benzoate 1,2-dioxygenase electron transfer component BenC (Paraburkholderia sabiae LMG 24235)
MEHRIALQFEDGVTRFITCRDNETLSDAAYRQKINIPLDCRDGACGTCRGFCESGSYDLP
EESYIEDALTPEDAAQGYVLACQTRPRSDCVIRVPASSAACKTGVARHEGTLANVQKLSD
STIEFSIDVDAPDQLTFLAGQYVNVEVPGSELSRSYSFSSAPGESRVSFVVRNVPEGKMS
TYLSDHAQAGQRVAFSGPYGAFYLRDAARPVLFLAGGTGIAPFLSMLDVLGAKEGAKQPV
RMVYGVTNDIDLVALPQLDAAQGKLPDFEYRTCVADPASEHALKGYVTAHVDPAWLNDGD
VDVYLCGPVAMVDAVRSWLGDIGVTPASFHYEKFSATNAA