Protein Info for QEN71_RS09295 in Paraburkholderia sabiae LMG 24235

Annotation: protein-L-isoaspartate(D-aspartate) O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 8 to 212 (205 residues), 202.3 bits, see alignment E=4.3e-64 PF01135: PCMT" amino acids 8 to 211 (204 residues), 196.4 bits, see alignment E=5.3e-62 PF05139: Erythro_esteras" amino acids 300 to 650 (351 residues), 429.9 bits, see alignment E=1.3e-132

Best Hits

KEGG orthology group: None (inferred from 59% identity to mno:Mnod_2056)

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (672 amino acids)

>QEN71_RS09295 protein-L-isoaspartate(D-aspartate) O-methyltransferase (Paraburkholderia sabiae LMG 24235)
METYDIEREQMVDAQIASRGILDPRVIEAMRTVPRHVFVPDDLTEFAYTDAPLPLEGGQT
ISQPYIVARMLEALELSPDDRVLDIGTGSGYAAAVAARLAAQVDSIERVGLLAESARATL
SAHGFVNVEVHDADGTLGWPSHAPFDAIVAAAGGPDVPLAWREQLAIGGRLVMPIGMQRE
RQRLVKVTRVSATQFDEEDLGDVHFVPLIGEQGWPELDDDANAHKPMPRAARDAVKAAMR
IEPLAGLIAAAARPLPAIDDPEFGEAFDFLADKRVVLLGESSHGTSEFYDARAAITRRLV
ERHGFRIVAAEADWPDAAVVDRYARLRPARGDQFPFQRFPAWMWRNEEFASFIGWLHSHN
ETLDTSQRCGFYGLDMYSLSASVAAVLDYLDRTDPDAASVARERYACLTPWQKDPQMYGR
AAYAEAFETCESAVIEQLQAMLRTRLDDAHDDAEMWFDATQNARLVASAERYYRLMYRSS
ADSWNLRDTHMFETLEHLLDCRGADSKAVVWAHNSHIGDAAATEMGRVRDELNVGRLCRE
HFGDEAALVGFGTHAGTVAAASDWDGPMQIKVVRPSRPDSVEHQFHASGQTRCVLDLTQR
AHPQLHERLREARLERFIGVIYRPETELHSHYAEASLSEQFDAFVWFDTTRAVEALATRA
TERAPDLYPFGV