Protein Info for QEN71_RS08050 in Paraburkholderia sabiae LMG 24235

Annotation: sarcosine oxidase subunit alpha family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 999 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 2 to 998 (997 residues), 1440.7 bits, see alignment E=0 PF13510: Fer2_4" amino acids 18 to 101 (84 residues), 84.5 bits, see alignment E=2.8e-27 PF00890: FAD_binding_2" amino acids 170 to 215 (46 residues), 24.6 bits, see alignment 9.6e-09 PF12831: FAD_oxidored" amino acids 170 to 214 (45 residues), 42 bits, see alignment (E = 5.3e-14) PF01134: GIDA" amino acids 170 to 228 (59 residues), 21.3 bits, see alignment 8.5e-08 PF07992: Pyr_redox_2" amino acids 170 to 431 (262 residues), 58.9 bits, see alignment E=3.9e-19 PF13450: NAD_binding_8" amino acids 173 to 211 (39 residues), 26.9 bits, see alignment (E = 3.2e-09) PF13738: Pyr_redox_3" amino acids 277 to 436 (160 residues), 30.5 bits, see alignment E=1.5e-10 PF17806: SO_alpha_A3" amino acids 517 to 599 (83 residues), 109.5 bits, see alignment E=5.4e-35 PF01571: GCV_T" amino acids 614 to 878 (265 residues), 249.4 bits, see alignment E=2.2e-77 PF08669: GCV_T_C" amino acids 904 to 990 (87 residues), 68.7 bits, see alignment E=2.2e-22

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 97% identity to bph:Bphy_3331)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (999 amino acids)

>QEN71_RS08050 sarcosine oxidase subunit alpha family protein (Paraburkholderia sabiae LMG 24235)
MSQNNRLGAGGRINRAIPLTFTFNGRTYQGFQGDTLASALLANGVHFVARSFKYHRPRGI
VTADVAEPNAVVQLERGAYTVPNARATEIELYQGLVASSVNAEPNLEHDHMAINQKFARF
MPAGFYYKTFMWPAKWWPKYEEKIREAAGLGKSPEVRDADRYDKCYAHCDVLVVGGGPTG
LAAAHAAAVSGARVILVDDQRELGGSLLSCKSEIDGHSAMSWVEKIEAELSRMPDVKILS
RSTAFGYQDHNLVTVTQRLTEHLPVSMRKGTRELLWKIRAKRVILATGAHERPIVFGNND
LPGVMLAGAVSTYVHRFGVLPGRNAVVFTNNDAGYQCALDMKACGASVTVVDPRAQGSGA
LQAAARRHGVKIMNNAAVMTAHGKQRVTSVEVVAYANGKTGAKQADLQCDLVAMSGGFSP
VLHLFAQSGGKAHWNDTKACFVPGKGMQPETSIGAAAGEFSLARGLRLAVDAGIEAVKSI
GYAVTRPQVPQVSEAVESPMQPLWLVGSRAEAARGPKQFVDFQNDVSAADILLAAREGFE
SVEHVKRYTAMGFGTDQGKLGNINGMAILADALGKTIPETGTTTFRPNYTPVTFGTFAGR
ELGDLLDPIRKTAVHEWHVENGAMFEDVGNWKRPWYFPLKGEDLHAAVKRECLAVRNSVG
ILDASTLGKIDIQGPDAAKLLNWMYTNPWSKLEVGKCRYGLMLDENGMVFDDGVTVRLAD
QHFMMTTTTGGAARVLTWMERWLQTEWPDMKVRLASVTDHWATFAVVGPKSRKVLQKVCS
DIDFANEAFPFMSYRNGTVAGVKARVMRISFSGELAYEVNVPANMGRAVWEALMAAGAEF
DITPYGTETMHVLRAEKGYIIVGQDTDGSVTPHDLGMGGLVAKTKDFLGRRSLTRSDTAK
EGRKQFVGLLPDDPQFVIPEGSQIVAGPFQGDTAPMLGHVTSSYYSPILNRSIALAVVKG
GLNKMGQSVTIPLSSGKQIAAKIASPVFYDTEGVRQHVE