Protein Info for QEN71_RS07265 in Paraburkholderia sabiae LMG 24235

Annotation: D-glycerate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 PF00389: 2-Hacid_dh" amino acids 5 to 316 (312 residues), 100.6 bits, see alignment E=8.8e-33 PF02826: 2-Hacid_dh_C" amino acids 105 to 284 (180 residues), 220.3 bits, see alignment E=1.9e-69 PF03446: NAD_binding_2" amino acids 145 to 252 (108 residues), 21.8 bits, see alignment E=2.5e-08

Best Hits

Swiss-Prot: 59% identical to GHRB_SERP5: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 93% identity to bph:Bphy_1251)

MetaCyc: 61% identical to glyoxylate reductase (Escherichia coli K-12 substr. MG1655)
Glyoxylate reductase (NADP(+)). [EC: 1.1.1.79]; Gluconate 2-dehydrogenase. [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.215, 1.1.1.79

Use Curated BLAST to search for 1.1.1.215 or 1.1.1.43 or 1.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (322 amino acids)

>QEN71_RS07265 D-glycerate dehydrogenase (Paraburkholderia sabiae LMG 24235)
MKRKIVAYKPLPDDVLAYLQQHAEVVQADAAQHDAFVAALNDADGAIGASVKITPAMLDG
ATKLKALSTISVGYDNFDVADLTKRGIVLAHTPDVLTESTADTVFSLILASARRVVELAN
FVKAGEWKASIGPELYGVDVQGKTLGIVGLGRIGGAVARRAALGFNMKVLYTNRSANAEA
EERYGARRVDLDELLATSDFVCLQVPLTPDTRHMIGANELRKMKKSAILINASRGQTVDE
YALIEALQSGTIHGAGLDVFDKEPLDPNSPLLKMNNVVALPHIGSATHETRHAMARCAAE
NLVGALDGTLKINIVNRDVLSQ