Protein Info for QEN71_RS06405 in Paraburkholderia sabiae LMG 24235

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 PF01479: S4" amino acids 485 to 529 (45 residues), 39.8 bits, see alignment 3.1e-14 PF00849: PseudoU_synth_2" amino acids 546 to 696 (151 residues), 53.8 bits, see alignment E=2.7e-18 TIGR00093: pseudouridine synthase" amino acids 587 to 726 (140 residues), 111.7 bits, see alignment E=1.5e-36

Best Hits

KEGG orthology group: K06182, ribosomal large subunit pseudouridine synthase F [EC: 5.4.99.12] (inferred from 76% identity to bph:Bphy_1341)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (732 amino acids)

>QEN71_RS06405 pseudouridine synthase (Paraburkholderia sabiae LMG 24235)
MRVKLTAKHPRPASSERAPVRSGSSTARKPTRAAGPADKPAAPRKPRDTATSGDAPRRPA
ARSNAASAPAERRGPSAGARSGASSPRSGDRPARAAGAGSRERGESGSYERRPAGDRPAR
GEGAGFRERGDSGSFERRRPAGDRPAHGEGAGFRERGDSGSFERRRPAGDRPARGEGAGF
RERGDSGSFERRRPAGDRPARGEGAGFRERGDPGSSERRRPAGDRPARGEGAGSRERSNS
GSFERRPFGDRPARREGGPAGTGFRRDSDARSDRRAPSDRPPRGDRPAGSGDRTPRFSRD
DDARGAPRAPRDNDDSRRAPRTARGAENAPTRERTERRYRDDASESRTARAPRGERSSEA
RGERTYARPARASYEDRGAAPERRPRGEGAPRRSRSDDTEARSERTYAKPVKAAYADRAS
DAPPKAAPAPRATKAKRDARSIEDSFDRPARFERPAEKTSRKPRPAAAPRQRDEGEFNDA
PGNLRLSKLMSELGLCSRREADEWIEKGWVTVDGVVVDTLGTKVRPDADIQIDPAAQAAQ
QKQVTILVHKPIGYVSGQAEDGYEPAVTLVKPENRWDGDHTDIRFSALHLRQLAPAGRLD
IDSTGLLVLTQDGRIAKQLIGGHSEIDKEYLVRVSYGDVETDVEHAFPAEELAKLRHGLE
LDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLHVVGLKRVRMGQVMLGALPP
GQWRYLGPDESF