Protein Info for QEN71_RS06270 in Paraburkholderia sabiae LMG 24235

Annotation: isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01346: isocitrate lyase
  • amino acids 8 to 252 (245 residues), 371.4 bits, E=3.3e-115
• PF00463: ICL
  • amino acids 12 to 252 (241 residues), 195.5 bits, E=1.4e-61
  • amino acids 253 to 425 (173 residues), 152.2 bits, E=1.9e-48
• PF13714: PEP_mutase
  • amino acids 78 to 276 (199 residues), 54.1 bits, E=1.7e-18

Best Hits

Swiss-Prot: 70% identical to ACEA_BACHD: Isocitrate lyase (aceA) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 98% identity to bph:Bphy_1368)

MetaCyc: 69% identical to isocitrate lyase (Escherichia coli K-12 substr. MG1655)
Isocitrate lyase. [EC: 4.1.3.1]

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1)" in subsystem Serine-glyoxylate cycle (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.1

Protein Sequence (434 amino acids)

>QEN71_RS06270 isocitrate lyase (Paraburkholderia sabiae LMG 24235)
MTRNEQAKQLQQQWETDSRWNGIKRGFTAEDVVRLRGSVQPEHTLARRGAERLWADMHKD
PFVNALGALTGNQAMQQVKAGLKAIYLSGWQVAGDANVAGEMYPDQSLYPANSVPLVVKR
INNTLTRADQIQWSEGKNPGDEGYIDYFAPIVADAEAGFGGVLNAFELMKAMIEAGAAGV
HFEDQLASVKKCGHMGGKVLVPTRENVAKLTAARLAADVLGVPTVLIARTDAEAADLMTS
DVDDNDKPFLTGERTVEGFFRTKPGLDQAVSRGLAYAPYADLIWCETGKPDLEYAKKFAE
AIHKEFPGKMLSYNCSPSFNWKKNLDDATIAKFQKELGAMGYKFQFITLAGFHALNYSMF
NLAHGYARQQMSAFVELQQAEFAAAEKGFTAVKHQREVGTGYFDAVTQTVERDASTTALH
GSTEDEQFFDKKVA