Protein Info for QEN71_RS05605 in Paraburkholderia sabiae LMG 24235

Annotation: glycine betaine/L-proline transporter ProP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 transmembrane" amino acids 46 to 63 (18 residues), see Phobius details amino acids 71 to 94 (24 residues), see Phobius details amino acids 106 to 125 (20 residues), see Phobius details amino acids 130 to 153 (24 residues), see Phobius details amino acids 175 to 196 (22 residues), see Phobius details amino acids 206 to 226 (21 residues), see Phobius details amino acids 265 to 286 (22 residues), see Phobius details amino acids 301 to 319 (19 residues), see Phobius details amino acids 331 to 350 (20 residues), see Phobius details amino acids 356 to 378 (23 residues), see Phobius details amino acids 390 to 414 (25 residues), see Phobius details amino acids 425 to 443 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 38 to 451 (414 residues), 160.5 bits, see alignment E=6.7e-51 PF07690: MFS_1" amino acids 40 to 385 (346 residues), 122.1 bits, see alignment E=2.6e-39

Best Hits

Swiss-Prot: 51% identical to OUSA_DICD3: Glycine betaine/proline/ectoine/pipecolic acid transporter OusA (ousA) from Dickeya dadantii (strain 3937)

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 96% identity to bph:Bphy_1491)

MetaCyc: 51% identical to osmolyte:H+ symporter ProP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-29; TRANS-RXN-29A

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (489 amino acids)

>QEN71_RS05605 glycine betaine/L-proline transporter ProP (Paraburkholderia sabiae LMG 24235)
MTASSNGFWRHKNNDNSLTLDDITVVDNSLLKRAVGAMALGNAMEWFDFGVYSYIAVTLG
KVFFPSSSPSAQLIATFGTFAAAFLVRPIGGMVFGPLGDRIGRQRVLAMTMIMMALGTFA
IGLIPSYGSIGILAPALLLIARLVQGFSTGGEYGGAATFIAEFATDRRRGFAGSFLEFGT
LVGYILGAGTVAVLTASLSQEALLSWGWRVPFFIAGPLGLVGLYIRMKLEETPAFKKEAE
AREADEKSRPKQSFMELLAQQWKPLLQCVGLVLIFNVTDYMALSYLPSYLSATLHFNESH
GLFLVLAVMVLMMPMTLYAGHLSDKIGRKPVMLLGCVGLFALSIPALLLIRTGAVLPVFG
GLLILGALLSTFTGVMPSSLPALFPTKIRYGALAIGFNVSVSLFGGTTPLVTAWLVDRTG
NLMMPAYYLMGASIIGIVSVLALRETARKPLLGSGPCVATRAEAHAVLRGEREAAELEEA
YTSAVTARA