Protein Info for QEN71_RS05300 in Paraburkholderia sabiae LMG 24235

Annotation: phenylalanine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 810 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 809 (809 residues), 777.3 bits, see alignment E=9.4e-238 PF01588: tRNA_bind" amino acids 45 to 151 (107 residues), 81.1 bits, see alignment E=1.4e-26 PF03483: B3_4" amino acids 219 to 391 (173 residues), 217.3 bits, see alignment E=2.8e-68 PF03484: B5" amino acids 410 to 475 (66 residues), 83.9 bits, see alignment E=1.9e-27 PF17759: tRNA_synthFbeta" amino acids 478 to 691 (214 residues), 210.7 bits, see alignment E=4.6e-66 PF03147: FDX-ACB" amino acids 707 to 809 (103 residues), 71.7 bits, see alignment E=1.4e-23

Best Hits

Swiss-Prot: 87% identical to SYFB_BURTA: Phenylalanine--tRNA ligase beta subunit (pheT) from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 97% identity to bph:Bphy_1553)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (810 amino acids)

>QEN71_RS05300 phenylalanine--tRNA ligase subunit beta (Paraburkholderia sabiae LMG 24235)
MQFPESWLRTFVDPQLTTAELSHALTMAGLEVEDLRPAAPPTSKIVVGRVLEVVKHPDAD
KLNVCQVDAGTGATLNIVCGAPNVAPGIKVPVALVGAQLPPAEEGGAPFAIKLSKLRGVQ
SEGMLCSARELKLSEDHSGLLILPEDTPIGQDIRETLNLDDTVFEIKLTPNKADCLSVFG
VARETAAITGAPLRPLEIKPVEVKLNETLPVKISAPDLCGRFSGRVIRGVNARAKSPAWM
VERLERSGQRSISALVDISNYVMLELGRPSHVFDLDKIHGSMDVRWGKRGESLKLLNGNT
VEVDETVGVIADDQHIESLAGIMGGDSTAVTLDTTNIYLEAAFWWPDSIRGRSRRYNFST
DAGHRFERGVDYSTTVEHIERITQLILDICGGEAGPVDDQIVNVPKRAPVKMRVARANRI
IGVAISADEIAQIFTRLGLPFERAGDDFLVTPPPYRFDIEIEEDLIEEVARIYGFEKIPA
RPPVARSEMRRTNETQRSIHTLRHALAARDYAETVNFSFVDAEWEQDFAGNDKPVKLLNP
IASQLSVMRTTLFGSLINVLRHNLNRRADRIRVFEAGRVFLQDASVKAGELAVEGFAQPK
MIGALAYGPVVEEQWGAATRAVDFFDVKGDLEALLAPAVARFVKAEHPALHPGRSARIEL
DGRAIGWIGELHPRWMQKYDLPHAPIVFEVEAEALMQRALPSPSEVSKFPPVRRDIAIVV
DQKIEVQALFDEMQKALSEEACKTIQRVALFDEFRAKSNTSGGLAAHEKSLAFRVTLQDT
GGTLQDETVDLAIQTLVDRLARVYGARLRG