Protein Info for QEN71_RS03995 in Paraburkholderia sabiae LMG 24235

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 979 PF13510: Fer2_4" amino acids 47 to 121 (75 residues), 86.7 bits, see alignment 3.8e-28 PF10588: NADH-G_4Fe-4S_3" amino acids 128 to 167 (40 residues), 60.4 bits, see alignment (E = 4.3e-20) PF13237: Fer4_10" amino acids 189 to 248 (60 residues), 25.8 bits, see alignment 3.8e-09 PF12838: Fer4_7" amino acids 193 to 250 (58 residues), 32 bits, see alignment 6.3e-11 PF00037: Fer4" amino acids 233 to 250 (18 residues), 23.9 bits, see alignment (E = 1.2e-08) PF04879: Molybdop_Fe4S4" amino acids 265 to 316 (52 residues), 57.8 bits, see alignment 3.8e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 269 to 944 (676 residues), 946.2 bits, see alignment E=4.1e-289 PF00384: Molybdopterin" amino acids 321 to 738 (418 residues), 239.5 bits, see alignment E=2.5e-74 PF01568: Molydop_binding" amino acids 836 to 941 (106 residues), 101.9 bits, see alignment E=9.5e-33

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 97% identity to bph:Bphy_0727)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (979 amino acids)

>QEN71_RS03995 formate dehydrogenase subunit alpha (Paraburkholderia sabiae LMG 24235)
MSDPITFKSGGCGSGNCACKAGALRNERGPLDDTDYGTPLRHSDVDVTLEIDGQMISVPA
GTSVMRAAAEAGVNIPKLCATDSLEPFGSCRLCLVEIEGRRGYPASCTTPVEAGMKVRTQ
TDRLQGLRRNVMELYISDHPLDCLTCPANGNCELQDMAGVTGLREVRYGYDGENHLKDQK
DESNPYFTYDPSKCIVCNRCVRACEETQGTFALTITGRGFESRVAASESQPFMESECVSC
GACVAACPTATLSEKSIVMMGQGEHSVVTTCAYCGVGCSFKAEMKGNTVVRMVPHKNGQA
NEGHACVKGRFAWGYATHKDRIKKPMIRAKITDPWREVSWEEALSYAASEFRRIQDKYGR
DSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKTTLGESAGTQTF
ASVDKSDVIIVIGANPTDGHPVFGSRLKRRVRGGAKLIVIDPRRIDIVDTAHVKAEHHLQ
LRPGTNVAMVTSLAHVIVSEGLLNEQFIAERCEDRAFQHWRDFVALPENSPEMMESVTGV
PAQQVREAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMATGNIGREGVGVNPLR
GQNNVQGSCDMGSFPHELPGYRHISDTVTRTLFEGEWGVTLQPEPGLRIPNMFDAALHGT
FMGLYCQGEDIVQSDPNTQHVGAALSSMECIVVQDIFLNETAKYAHVLLPGSTFLEKDGT
FTNAERRISRVRKVMPPMPGYSDWEVTILLARALGYEMNYTHPSQIMDEIARLTPTFHGV
SYEKLDKLGSIQWPCNEHAPEGTPTMHIDEFVRGKGKFVITKYVATPEKVNQKFPLILTT
GRILSQYNVGAQTRRTENSRWHEEDRLEIHPHDAEDRGIKDDDWVGIESRAGYTVLRAKV
ADRMQPGVVYTTFHFPESGANVITTDSSDWATNCPEYKVTAVQVMPVEQPSQWQKDYSRF
NDEQLEHLSKREMATTSGK