Protein Info for QEN71_RS01720 in Paraburkholderia sabiae LMG 24235

Annotation: bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 275 (275 residues), 274.7 bits, see alignment E=4.1e-86 PF01149: Fapy_DNA_glyco" amino acids 1 to 117 (117 residues), 128.1 bits, see alignment E=4.2e-41 PF06831: H2TH" amino acids 136 to 226 (91 residues), 75.2 bits, see alignment E=5e-25 PF06827: zf-FPG_IleRS" amino acids 248 to 275 (28 residues), 44.1 bits, see alignment (E = 2.1e-15)

Best Hits

Swiss-Prot: 99% identical to FPG_PARP8: Formamidopyrimidine-DNA glycosylase (mutM) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 99% identity to bph:Bphy_0319)

MetaCyc: 49% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (276 amino acids)

>QEN71_RS01720 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase (Paraburkholderia sabiae LMG 24235)
MPELPEVEVTRRGIEPYVAGRRVERVDVRTPALRWPIPPGFARLLHGRLVHKVERRGKYL
LFEIDEGWFIVHLGMTGTLRVLRNVPHPPAAAKHDHVDWIFDDFILRFRDPRRFGAVLWH
PRSAGDVLDHPLLADLGVEPFAPSFSGALLHRKTRGRKVSVKQALLAGDMVVGVGNIYAS
ESLFRAGIRPTTPAGRISLVRYDLLADAVRVTLAAAIEKGGSTLRDFVGSNGESGYFQLD
YFVYDRAGLPCRVCGTPIKQIVQGQRSTYYCPTCQR