Protein Info for QEN71_RS01595 in Paraburkholderia sabiae LMG 24235

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 862 PF00358: PTS_EIIA_1" amino acids 19 to 142 (124 residues), 149.3 bits, see alignment E=1.1e-47 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 19 to 139 (121 residues), 130 bits, see alignment E=6.8e-42 PF00381: PTS-HPr" amino acids 186 to 262 (77 residues), 59.6 bits, see alignment E=6.3e-20 TIGR01003: phosphocarrier, HPr family" amino acids 186 to 261 (76 residues), 48.7 bits, see alignment E=8.3e-17 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 302 to 854 (553 residues), 475.3 bits, see alignment E=3.8e-146 PF05524: PEP-utilisers_N" amino acids 303 to 422 (120 residues), 80.4 bits, see alignment E=3e-26 PF00391: PEP-utilizers" amino acids 447 to 520 (74 residues), 72.6 bits, see alignment E=4.3e-24 PF02896: PEP-utilizers_C" amino acids 548 to 831 (284 residues), 337.4 bits, see alignment E=1.7e-104

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 93% identity to bph:Bphy_0295)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) / PTS system, glucose-specific IIA component" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (862 amino acids)

>QEN71_RS01595 phosphoenolpyruvate--protein phosphotransferase (Paraburkholderia sabiae LMG 24235)
MRRVEESRLKSHSEGHIVLLAPMTGPVVPLANVPDPVFSGGMFGDGIGIDPLEGKLVAPC
DGVVMHLARTGHAVTITTEEGAEILLHIGIDTVELNGRGFAPMIEQGATVRSGDVLIEFD
QDVVARNAPSLVSVIAIANSDAFEIVERADHGVLKAGQSPLLTLRVKDGAAVAASREASS
VSEEARQTIVLDHAGGLHARPAARAREAARGLDARVEVRFDGKKAAIESVVGLLGLGAGQ
GATVEIVGIGAQAQAAVDAVVRELTREAHGEAEEKPARQMSPAPVTAVRPQGAESLAPNT
LAGVCAAPGIAVGKLVRWDDADIDPPETTGNTPAAESRQLDKALAAVDAELNTTVRDASQ
RGAHGEAGIFAVHRVLLEDPTLIDAARDLISLGKSAGFAWRETIRTQIGVLSKVDDALLA
ERAADLRDIEKRVLRALGYSNAAARSLPDEAVLSADEFTPSDLSSLDRKRVTALVMARGG
ATSHAAIIARQAGIPALVAMGDALHTIADGTQVVVDATAGRLEYAPSALDVERARNERER
LAGEREANRRTSKQAAATRDGRAIEVAANIATLDDATTAVDNGADAVGLLRTELLFIHRQ
AAPTVDEHRESYQSIVEALQGRTAIIRTLDVGADKEVDYLTLPPEPNPALGLRGIRLAQV
RPDLLEDQLRGLLAVQPVGKVRILLPMVTDAGELVRLRKRIDELAVELGRTEKIEVGVMI
EVPSAALLADQLSKHADFLSIGTNDLTQYTLAMDRCQPDLAAQADGMHPAVLRLVSAAVQ
GAEKHGKWVGVCGALAGDPLAVPLLVGLGVTELSVDPVSVPGIKARVRNLDYQLCRQRAQ
DALALESAQAVRALSREIWPQD