Protein Info for Psyr_4864 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: extracellular solute-binding protein, family 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF13416: SBP_bac_8" amino acids 42 to 328 (287 residues), 104.6 bits, see alignment E=1.3e-33 PF01547: SBP_bac_1" amino acids 43 to 297 (255 residues), 54.1 bits, see alignment E=3.8e-18 PF13343: SBP_bac_6" amino acids 76 to 308 (233 residues), 62.5 bits, see alignment E=6.7e-21

Best Hits

Swiss-Prot: 68% identical to SPUE_PSEAE: Spermidine-binding periplasmic protein SpuE (spuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 100% identity to psb:Psyr_4864)

MetaCyc: 55% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLT1 at UniProt or InterPro

Protein Sequence (364 amino acids)

>Psyr_4864 extracellular solute-binding protein, family 1 (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSISILSKALLAAAGLTLTLGAQAESTVHIYNWSDYIGKTTLADFEATTGIKPMYDVFDS
NETLEAKLLAGRTGYDVVVPSNHFLGKQIKAGAFQKLDKSLLPNYKNLDPALLKRLEKND
PGNQYAVPYLWGTNGIGYNVEKVKAVLGVDKIDSWAVLFEPENIKKLSSCGVAFLDSADE
MLPAVLNYLGLDPNSTKEADYKEAEAKLMAIRPYVTYFHSSKYITDLANGDICVAAGFSG
DVFQAKARAEEAGKGVKLAYSIPKEGGNLWFDMLAIPADSKNIKQASAFINYMLDPRVIA
KVSDEVGYANPNPASGEFMDQKIRTDAAVYPPQEVLDRLFVNSELPLKVQRLMTRSWTKV
KSGE