Protein Info for Psyr_4606 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Outer membrane autotransporter barrel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00657: Lipase_GDSL" amino acids 31 to 320 (290 residues), 55.8 bits, see alignment E=1.1e-18 PF03797: Autotransporter" amino acids 371 to 620 (250 residues), 157.6 bits, see alignment E=7.9e-50

Best Hits

Swiss-Prot: 62% identical to ESTA_PSEAE: Esterase EstA (estA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12686, outer membrane lipase/esterase (inferred from 100% identity to psb:Psyr_4606)

Predicted SEED Role

"Phospholipase/lecithinase/hemolysin"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZMI6 at UniProt or InterPro

Protein Sequence (640 amino acids)

>Psyr_4606 Outer membrane autotransporter barrel (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTKTSRRWPFAACLLSLACGTAAAAPYSTMVVFGDSLADAGQFPDTAGPRGSTLRFTNRV
GPTYQDGSGEVFNLNSSTLIGRMLGVPAGDLAASTSPVNAALGAPDGNNWAVGGYRTDQI
LDSINSQSTVVDPNTGTLLRSRTGYLPANGFRADPNALYYLTGGGNDFLQGRVLSASSAA
QAANQLADSAQALQQAGARYIMVWLLPDIGKTPALSGSPLASATSALSAGFNQQLVSRLA
QINAQIIPLNVPLLINEVLAEPARFGFDPNENLVSTCFSGNSCRESTTNGRSSATPNPNR
LFFNDRVHPTEAGQRLLADYAYSLLSAPWEISLLPEMANGTLRMHQDEIRAQWLSDWGNW
QGIGQWQSMLSAGGQKMDFDAQDSSADADGRGYNLTIGGSYRFAENWRTGVVAGAYRQSL
EAGPRDSDYKLNSYIATAFLQYQANHWWGDLAVSGGKLDYENAERKFALGVSEGQEKGDT
DGEMWAVSGRVGFDIAGPTSRWHLSPFVSADYAHINVDGYSEKGDRSTALTFSDQTRKSR
RAGVGVQGKFQVTPSTQVWGEVAHEREFETDQQDVTMALNSVQSVGFTLQGYTPQRDLNR
ATLGVSQKLTQDLTLRGNYNWRKNDDVTQQGVNVALSLSF