Protein Info for Psyr_4550 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: translation elongation factor 1A (EF-1A/EF-Tu)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 TIGR00485: translation elongation factor Tu" amino acids 1 to 396 (396 residues), 725.5 bits, see alignment E=1.3e-222 PF00009: GTP_EFTU" amino acids 11 to 204 (194 residues), 205.4 bits, see alignment E=9.5e-65 TIGR00231: small GTP-binding protein domain" amino acids 13 to 147 (135 residues), 52.8 bits, see alignment E=4e-18 PF03144: GTP_EFTU_D2" amino acids 228 to 296 (69 residues), 63.8 bits, see alignment E=2.5e-21 PF03143: GTP_EFTU_D3" amino acids 301 to 395 (95 residues), 140.5 bits, see alignment E=3.5e-45

Best Hits

Swiss-Prot: 100% identical to EFTU_PSEU2: Elongation factor Tu (tuf) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K02358, elongation factor Tu (inferred from 100% identity to psb:Psyr_4550)

Predicted SEED Role

"Translation elongation factor Tu" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZMP2 at UniProt or InterPro

Protein Sequence (397 amino acids)

>Psyr_4550 translation elongation factor 1A (EF-1A/EF-Tu) (Pseudomonas syringae pv. syringae B728a ΔmexB)
MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAAVEFDKIDSAPEEKARG
ITINTAHVEYKSLIRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
LLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLSTYDFPGDDTPIIIGSARMALE
GKDDNEMGTTAVRKLVETLDSYIPEPVRVTDKPFLMPIEDVFSISGRGTVVTGRIERGIV
KVQDPLEIVGLRDTTVTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPG
SVKPHTQFEAEIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSCELPEGVEMVMPGDNV
KVSVTLIKPIAMEDGLRFAIREGGRTVGAGVVAKIIA