Protein Info for Psyr_4387 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Glycosyl transferase, family 51:Penicillin-binding protein, transpeptidase:Penicillin-binding, C-terminal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 782 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details signal peptide" amino acids 31 to 31 (1 residues), see Phobius details TIGR02073: penicillin-binding protein 1C" amino acids 43 to 780 (738 residues), 790.6 bits, see alignment E=8.8e-242 PF00912: Transgly" amino acids 62 to 228 (167 residues), 177.7 bits, see alignment E=2.4e-56 PF00905: Transpeptidase" amino acids 304 to 420 (117 residues), 35.7 bits, see alignment E=8.9e-13 PF06832: BiPBP_C" amino acids 696 to 778 (83 residues), 67.6 bits, see alignment E=1.1e-22

Best Hits

Swiss-Prot: 60% identical to PBPC_ECOLI: Penicillin-binding protein 1C (pbpC) from Escherichia coli (strain K12)

KEGG orthology group: K05367, penicillin-binding protein 1C [EC: 2.4.1.-] (inferred from 100% identity to psb:Psyr_4387)

MetaCyc: 60% identical to peptidoglycan glycosyltransferase PbpC (Escherichia coli K-12 substr. MG1655)
Peptidoglycan glycosyltransferase. [EC: 2.4.1.129]

Predicted SEED Role

"Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C" in subsystem Peptidoglycan Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-, 2.4.1.129, 2.4.2.-

Use Curated BLAST to search for 2.4.1.- or 2.4.1.129 or 2.4.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZN55 at UniProt or InterPro

Protein Sequence (782 amino acids)

>Psyr_4387 Glycosyl transferase, family 51:Penicillin-binding protein, transpeptidase:Penicillin-binding, C-terminal (Pseudomonas syringae pv. syringae B728a ΔmexB)
MKPEFLLRLPRTFKWLAGAIMLLIALLWLADRLWPLPLPRDDLARVVLAEDGTPLWRFAD
TNGVWRYPVSNAQVSPYYLEALLTYEDRWFYSHPGVNPLALLRATWQNLSGARVVSGGST
LSMQVARLLDPHSRTLSGKFRQLWRTLQLEWHLSKDQILSLYLNRAPFGGTLQGVAAASW
AYLGKSPQNLTRAEAALLAVLPQAPSRLRPDRHPQRAQQARDKVLRRLAEFQVWPRASVN
EALEEPLWLAPRQEPSLAPLLARRLNRPNSPPLIRTTLDASLQRRMEDLLMGWRARLPER
TSAAILVVEAENMAVRAYVGSVDINDAKRFGHVDMVTALRSPGSTLKPFLYGMAMDAGLI
HSESLMQDVPRRYGDYRPGNFSSGFGGPVAVSSALSMSLNLPAVQLLEVYGPKRFAAELR
NGGVPLSLPPLAEPSLALILGGAGSRLEDLVTGYSAFARGGRSADVRLQPQDRLRERRMM
SPGAAWIIRRILSGQSRPDIDPRAELVQRPQLAWKTGTSYGFRDAWAIGVGPRFLVGVWI
GRPDGTPVPGQFGLASAAPLMLQVHDVLVNRDSQRGIAAPMQPVPLNVGVAAICWPLGQP
MSKSAPNCRRQRFAWTLDGTTPPTLQAADQPLGLGLQERVWINAKGLRVAANCPDARAQD
IALWPAPLEPWLPRAERRDARLPPADPDCPPQNLNLAPPLSIVGVREGDNLRLPAASRQA
LRLTLSALGGSGHRWWFIDGKPLADTDTRQDFTPTLSKPGRYQLSVLDESGQTARVEFSV
VE