Protein Info for Psyr_4253 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: bacterial peptide chain release factor 3 (bRF-3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 527 TIGR00503: peptide chain release factor 3" amino acids 3 to 526 (524 residues), 862.8 bits, see alignment E=8e-264 PF00009: GTP_EFTU" amino acids 11 to 274 (264 residues), 162.7 bits, see alignment E=2e-51 TIGR00231: small GTP-binding protein domain" amino acids 13 to 146 (134 residues), 59.6 bits, see alignment E=3.3e-20 PF03144: GTP_EFTU_D2" amino acids 311 to 377 (67 residues), 46.7 bits, see alignment E=8.5e-16 PF16658: RF3_C" amino acids 384 to 511 (128 residues), 157.3 bits, see alignment E=4.5e-50 PF14492: EFG_III" amino acids 396 to 459 (64 residues), 27.8 bits, see alignment E=5.4e-10

Best Hits

Swiss-Prot: 100% identical to RF3_PSEU2: Peptide chain release factor 3 (prfC) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 100% identity to psb:Psyr_4253)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNI9 at UniProt or InterPro

Protein Sequence (527 amino acids)

>Psyr_4253 bacterial peptide chain release factor 3 (bRF-3) (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTQQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAISVAGTVKSRKSDRHATSDWMEM
EKQRGISITTSVMQFPYRDHMINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPR
TIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVLKIKAAPITWPIGCYRDFKGV
YHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFVEQLELVQGACHEFNQ
QEFIDGQLTPVFFGTALGNFGVDHVLDAVVNWAPKPLARVANERTVEPAEEKFSGFVFKI
QANMDPKHRDRIAFMRICSGRYDKGMKMRHVRLGKDVRIGDALTFFSSEREQLEEAYAGD
IIGLHNHGTIQIGDTFTEGEALGFTGIPHFAPELFRRVRLKDPLKSKQLRQGLQQLAEEG
ATQVFFPQRSNDIILGAVGVLQFDVVASRLKEEYKVECAYEPITVWSARWIDCDDKKKLE
EFENKAVENLAVDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH