Protein Info for Psyr_4143 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: HAD-superfamily hydrolase, subfamily IIIA:Phosphatase YrbI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 169 TIGR01670: 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family" amino acids 7 to 159 (153 residues), 175.1 bits, see alignment E=4.8e-56 PF08282: Hydrolase_3" amino acids 82 to 143 (62 residues), 32.1 bits, see alignment E=1.5e-11

Best Hits

Swiss-Prot: 52% identical to KDSC_YERPE: 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC (kdsC) from Yersinia pestis

KEGG orthology group: K03270, 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (KDO 8-P phosphatase) [EC: 3.1.3.45] (inferred from 100% identity to psb:Psyr_4143)

MetaCyc: 48% identical to 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase monomer (Escherichia coli BL21(DE3))
3-deoxy-manno-octulosonate-8-phosphatase. [EC: 3.1.3.45]

Predicted SEED Role

"3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45)" in subsystem KDO2-Lipid A biosynthesis (EC 3.1.3.45)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNU9 at UniProt or InterPro

Protein Sequence (169 amino acids)

>Psyr_4143 HAD-superfamily hydrolase, subfamily IIIA:Phosphatase YrbI (Pseudomonas syringae pv. syringae B728a ΔmexB)
MQRGKAIKLAVFDVDGVLTDGRLYFMEDGSEIKTFNTLDGQGIKMLIASGVTTAIISGRK
TAIVERRAKSLGIEHLFQGREDKLVVLDKLLAELNLSYEQVAYLGDDLPDLPVIRRVGLG
MAVANAAGFVREHAHGITRARGGEGAAREFCELILSAQGNLEAAHSAYL