Protein Info for Psyr_3248 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Helix-turn-helix, Fis-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00158: Sigma54_activat" amino acids 26 to 189 (164 residues), 241.7 bits, see alignment E=5.4e-76 PF14532: Sigma54_activ_2" amino acids 29 to 194 (166 residues), 70.9 bits, see alignment E=2e-23 PF07728: AAA_5" amino acids 47 to 166 (120 residues), 33.6 bits, see alignment E=5.5e-12

Best Hits

Swiss-Prot: 85% identical to SFNR_PSEPF: Sigma54-dependent transcriptional regulator SfnR (sfnR) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_3248)

Predicted SEED Role

"Alkanesulfonates-binding protein" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization or Putative sulfate assimilation cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRE2 at UniProt or InterPro

Protein Sequence (368 amino acids)

>Psyr_3248 Helix-turn-helix, Fis-type (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSGQLLTLPHSPALATSIRATAQVFEDPKSRALLAHVRQVAPSDASVLIIGETGTGKELV
ARHVHELSARRDKPFVAVNCGAFSENLIEAELFGHEKGAFTGAISAKAGWFEEANGGTLF
LDEIGDLPMTLQVKLLRVLQEREVVRLGSRKSVPIDVRVLAATNVQLEKAINAGHFREDL
YYRLDVVNLELSPLRERPGDILPLARHFIEVYSQRLRHGPVRISPEAEHKLKTYSWPGNI
RELENVIHHTLLVCREGVIQRDDLRMSNLRIERQDEHNPPDESAEQLLNRAFQKLFEEQA
GALHEKVEDTLLRTAYRFCHHNQVHTASLLGLTRNVTRTRLIKIGELAVNKRRPGENVQG
DRMLHLSV