Protein Info for Psyr_2523 in Pseudomonas syringae pv. syringae B728a ΔmexB
Annotation: DNA polymerase III, alpha subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to DNAE2_PSEU2: Error-prone DNA polymerase (dnaE2) from Pseudomonas syringae pv. syringae (strain B728a)
KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 100% identity to psb:Psyr_2523)Predicted SEED Role
"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.7
Use Curated BLAST to search for 2.7.7.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZTG0 at UniProt or InterPro
Protein Sequence (1031 amino acids)
>Psyr_2523 DNA polymerase III, alpha subunit (Pseudomonas syringae pv. syringae B728a ΔmexB) MTREYAELHCLSNFSFQRGASSARELFERALRHGYKALAITDECTLAGIVRAWQASKSTG LPLIVGSEMHIENGPKVVLLVENQAGYEALCKLITVARRRAGKGEYRVLREDFEPAPDGL LALWLPDLDGNAQACLAGGRWLRERFAERLWLGVGLHRGPDDEQRLADLLALAQSLGVPA VASGDVHMHARGRRALQDTMTAIRHHTTVAEAGHLLFANGERHLRPLDALSEHYPDWLLA ESVRIARRCTFDLGDLKYEYPHELVPKGQTSTSWLRELTERGVRRRWPGGLTPATRAQVE KELALIAEKKFDSYFLTVHDIVEFARSQHILCQGRGSAANSAVCYALGITELNPEQSNLL FERFISRERNEPPDIDVDFEHDRREEVIQYIFRRYGRGRAALTAVASTYHGSGAMRDVAK VLGLPPEQINALAEAFSRWSDSLPSPERLREYGFDADTPILKRVLALTGELIGFPRHLSQ HPGGFVISEHPLETLVPVENAAMADRTIIQWDKDDLDLVGLLKVDILALGMLSALRRTFD LVHLHRGQRWTLATLPGDDRKTYEMISRADTIGVFQIESRAQMAMLPRLRPEKFYDLVIE VAIVRPGPIQGDMVHPYLRRRNGEEDVTYPPKLESVFKRTLGVPLFQEQVMEVAILAADY TPGEADELRRAMAAWKRHGGLEPHRERLRTGMLKNGYEADFADRIFEQIKGFGSYGFPES HAASFALLTYASCWLKCHEPAAFTCALINSWPMGFYSPDQLLQDARRHHIEIRPVDVRYS DWDCSLEPLDHPDRTRNLAIRLGLRMVRSFREEDALRIEVARAKRPFVDATDLTLRAELD ARAAEALADSGALRGLIGHRHRARWEVAGVEAQRPLFDDLPSEETQVTLPLPTVAEDLVA DYTTLGTTLGPHPLALLRRQLAAKRFRSSQDLLHLENDRTLSVAGLVIGRQRPGTASGVT FVTLEDEFGMVNVVVWRDLAERQRKVLVGSQLLQVFGRLESKSGVRHLIAQRLYDLTPLL TGLDVRSRDFQ