Protein Info for Psyr_1331 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: succinyldiaminopimelate desuccinylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01246: succinyl-diaminopimelate desuccinylase
  • amino acids 11 to 381 (371 residues), 539.1 bits, E=2.6e-166
• PF01546: Peptidase_M20
  • amino acids 69 to 378 (310 residues), 145.6 bits, E=1.9e-46
• PF07687: M20_dimer
  • amino acids 183 to 290 (108 residues), 100.9 bits, E=3.8e-33

Best Hits

Swiss-Prot: 100% identical to DAPE_PSEU2: Succinyl-diaminopimelate desuccinylase (dapE) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01439, succinyl-diaminopimelate desuccinylase [EC: 3.5.1.18] (inferred from 100% identity to psb:Psyr_1331)

MetaCyc: 60% identical to succinyl-diaminopimelate desuccinylase (Escherichia coli K-12 substr. MG1655)
Succinyl-diaminopimelate desuccinylase. [EC: 3.5.1.18]

Predicted SEED Role

"N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 3.5.1.18)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.18

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

See Q4ZWU0 at UniProt or InterPro

Protein Sequence (383 amino acids)

>Psyr_1331 succinyldiaminopimelate desuccinylase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTAPADLSPTLQLACDLIRRPSVTPVDADCQTVMMQRLGDAGFKLEPMRIEDVDNFWATH
GTTDGPVLCFAGHTDVVPTGPLQNWQNDPFDALIDEHGMLCGRGAADMKGSLAAMLVAAE
RFVADHPDHKGSVAFLITSDEEGPAHHGTKAVVERLAARNERLDWCIVGEPSSTTLVGDV
VKNGRRGSLGATLTVRGKQGHVAYPHLAKNPIHLAAPALAELAAEHWDHGNDFFPPTSFQ
ISNLNAGTGATNVIPGDLVAVFNFRFSTESTVEGLQQRVADILDRHELDWHVDWALSGLP
FLTEPGALLDAVSSSIKSVTGRETKASTSGGTSDGRFIATLGTQVVELGPVNATIHQVNE
RILASDLDVLTEIYYQTLVKLLA