Protein Info for Psyr_1310 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: bacterial peptide chain release factor 2 (bRF-2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 PF03462: PCRF" amino acids 2 to 184 (183 residues), 179.1 bits, see alignment E=9.2e-57 TIGR00020: peptide chain release factor 2" amino acids 2 to 327 (326 residues), 491 bits, see alignment E=9.9e-152 PF00472: RF-1" amino acids 192 to 301 (110 residues), 146.4 bits, see alignment E=3.4e-47

Best Hits

Swiss-Prot: 70% identical to RF2_XANC5: Peptide chain release factor 2 (prfB) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K02836, peptide chain release factor 2 (inferred from 100% identity to psb:Psyr_1310)

Predicted SEED Role

"Peptide chain release factor 2; programmed frameshift-containing"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZWW1 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Psyr_1310 bacterial peptide chain release factor 2 (bRF-2) (Pseudomonas syringae pv. syringae B728a ΔmexB)
MNRELEDPNVWNNPAYAQELGRERSLLAQIVETLDEMSSGLADAKDLLLMSAEEEDQAAV
DDVAAEVERLRESLEKLEFRRMFSGDMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWA
DKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTS
FSAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQN
ERSQHANKDTAMKMLRARLYEQEVQKRNAASQALEDTKSDIGWGHQIRSYVLDASRIKDL
RTNIERSDCDKVLDGDIDEYLIASLKQGL