Protein Info for Psyr_0568 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Protein of unknown function UPF0079

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00150: tRNA threonylcarbamoyl adenosine modification protein YjeE
  • amino acids 1 to 124 (124 residues), 155.1 bits, E=4.7e-50
• PF02367: TsaE
  • amino acids 2 to 116 (115 residues), 130.2 bits, E=2.4e-42

Best Hits

Swiss-Prot: 59% identical to TSAE_SHIFL: tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE) from Shigella flexneri

KEGG orthology group: K06925, UPF0079 ATP-binding protein (inferred from 100% identity to psb:Psyr_0568)

MetaCyc: 59% identical to N6-L-threonylcarbamoyladenine synthase, TsaE subunit (Escherichia coli K-12 substr. MG1655)
RXN-14570 [EC: 2.3.1.234]

Predicted SEED Role

"TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

MetaCyc Pathways

• N⁶-L-threonylcarbamoyladenosine³⁷-modified tRNA biosynthesis (1/2 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.234

Sequence Analysis Tools

See Q4ZYY4 at UniProt or InterPro

Protein Sequence (143 amino acids)

>Psyr_0568 Protein of unknown function UPF0079 (Pseudomonas syringae pv. syringae B728a ΔmexB)
MMDFGARLAKVTEGLGVIFLDGDLGAGKTTLSRGLIRGFGHAGAVKSPTFTLVEPYEIGA
IKVFHFDLYRLVDPEELEFMGVRDYFDGDALCLIEWPQRGAGFLPKPDLTITIVPHGEGR
SVILSPLGSRGEQWCATLALEFK