Protein Info for PfGW456L13_953 in Pseudomonas fluorescens GW456-L13

Annotation: FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 759 PF13185: GAF_2" amino acids 16 to 154 (139 residues), 56 bits, see alignment E=1.6e-18 PF01590: GAF" amino acids 17 to 153 (137 residues), 64.9 bits, see alignment E=3.5e-21 PF13492: GAF_3" amino acids 18 to 155 (138 residues), 48 bits, see alignment E=5.1e-16 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 179 to 735 (557 residues), 451.4 bits, see alignment E=2.1e-139 PF05524: PEP-utilisers_N" amino acids 180 to 301 (122 residues), 96 bits, see alignment E=5.6e-31 PF00391: PEP-utilizers" amino acids 327 to 399 (73 residues), 64 bits, see alignment E=2.5e-21 PF02896: PEP-utilizers_C" amino acids 429 to 714 (286 residues), 344.5 bits, see alignment E=1.4e-106

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 97% identity to pba:PSEBR_a246)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QI95 at UniProt or InterPro

Protein Sequence (759 amino acids)

>PfGW456L13_953 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated (Pseudomonas fluorescens GW456-L13)
MLNTLRKIVQEVNSAKDLKAALGIIVLRVKEAMGSQVCSVYLLDPETNRFVLMATEGLNK
RSIGKVSMAPNEGLVGLVGTREEPLNLENAADHPRYRYFAETGEERFASFLGAPIIHHRR
VVGVLVIQQKERRQFDEGEEAFLVTMSAQLAGVIAHAEATGSIRGLGRQGKGIQEAKFVG
VPGSPGAAVGTAVVMLPPADLDVVPDKTITDIDAELGLFKTAIEGVRADMRTLSAKLATQ
LRPEERALFDVYLMMLDDAALGSEVTTVIKTGQWAQGALRQVVTDHVNRFELMDDAYLRE
RASDVKDLGRRLLAYLQEERQQTLVYPDNTILVSEELTPAMLGEVPEGKLAGLVSVLGSG
NSHVAILARAMGIPTVMGLVDLPYSKVDGIQMIVDGYHGEVYTNPSEVLRKQFAEVVEEE
KQLALGLDALRDLPCVTVDGHRMPLWVNTGLLADVARAQKRGAEGVGLYRTEVPFMINQR
FPSEKEQLAIYREQLSAFHPQPVTMRTLDIGGDKSLSYFPIKEDNPFLGWRGIRVTLDHP
EIFLVQTRAMLKASEGLNNLRILLPMISGTHELEEALHLIHRAWGEVRDEGCDVPMPPIG
VMIEIPAAVYQTKELARQVDFLSVGSNDLTQYLLAVDRNNPRVADLYDYLHPAVLQALQN
VVRDAHAEGKPVSICGEMAGDPAAAVLLMAMGFDSLSMNATNLPKVKWMLRQINLSKARE
LLAELMTIDNPQVIHSSLQLALKNLGLARMINPAAIKPL