Protein Info for PfGW456L13_724 in Pseudomonas fluorescens GW456-L13

Annotation: ATP-dependent DNA helicase UvrD/PcrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 PF00580: UvrD-helicase" amino acids 12 to 274 (263 residues), 294.8 bits, see alignment E=1.9e-91 PF13245: AAA_19" amino acids 16 to 257 (242 residues), 78.9 bits, see alignment E=1e-25 PF13361: UvrD_C" amino acids 279 to 618 (340 residues), 272.5 bits, see alignment E=1.7e-84 PF13538: UvrD_C_2" amino acids 558 to 614 (57 residues), 29.8 bits, see alignment 1.1e-10

Best Hits

Swiss-Prot: 65% identical to UVRD_ECOLI: DNA helicase II (uvrD) from Escherichia coli (strain K12)

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 97% identity to pfo:Pfl01_5644)

MetaCyc: 65% identical to DNA helicase II (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QF80 at UniProt or InterPro

Protein Sequence (727 amino acids)

>PfGW456L13_724 ATP-dependent DNA helicase UvrD/PcrA (Pseudomonas fluorescens GW456-L13)
MRDDLSLLLNSLNDAQRQAVAAPVGRQLVLAGAGSGKTRVLVHRIAWLIQVENASPHSIL
SVTFTNKAAAEMRQRIEQLMGINPAGMWVGTFHGLAHRLLRAHWQEAGLSQTFQILDSDD
QQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGLRPQHIQASGDLFLATMRGIYEAYE
AACLRAGVIDFSELLLRALDLWRDHPGLLAHYQKRFRHILVDEFQDTNAVQYAWLRLLAK
GGDSLMVVGDDDQSIYGWRGAKIENIYQYSEDFPDAETIRLEQNYRSTAGILKAANALIA
NNTGRLGKELWTDGGDGEAINLYAAFNEHDEARYVVETIESALKTGLSRSDIAILYRSNA
QSRVLEEALLRERIPYRIYGGQRFFERAEIKNAMAYMRLLEGRGNDAALERVINVPARGI
GEKTVEAIRDHARHSDVSMWEAMRQLVANKGLTGRAAGALGAFIELIENLAAKCMEMPLH
LMTQTVIEQSGLIAYHEAEKGEKGQARVENLEELVSAARNFENSEEDEDLTPLAAFLGHA
SLEAGDTQADEHEDSIQLMTLHSAKGLEFPYVFLVGMEEGLFPHKMSLEEPGRLEEERRL
AYVGITRAMQNLVMTFAETRRLYGSETYNKVSRFVREVPKGLIQEVRLSNSVSRPFGGNQ
SMSGSNLFSGSEIPQTGFSLGQTVRHSIFGDGVILNFEGAGAQARVQVNFSEGSKWLMLG
YAKLEAI