Protein Info for PfGW456L13_671 in Pseudomonas fluorescens GW456-L13

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 632 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 7 to 622 (616 residues), 913.3 bits, see alignment E=3.3e-279 PF12831: FAD_oxidored" amino acids 8 to 142 (135 residues), 32.7 bits, see alignment E=1.4e-11 PF01134: GIDA" amino acids 8 to 401 (394 residues), 572.8 bits, see alignment E=1e-175 PF21680: GIDA_C_1st" amino acids 462 to 556 (95 residues), 92.5 bits, see alignment E=5.9e-30 PF13932: GIDA_C" amino acids 561 to 616 (56 residues), 84.3 bits, see alignment 1e-27

Best Hits

Swiss-Prot: 98% identical to MNMG_PSEPF: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 98% identity to pfo:Pfl01_5741)

MetaCyc: 69% identical to 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QHW9 at UniProt or InterPro

Protein Sequence (632 amino acids)

>PfGW456L13_671 tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (Pseudomonas fluorescens GW456-L13)
VDFPSRFEVIVIGGGHAGTEAALASARMGVKTLLLTHNVETLGAMSCNPAIGGIGKSHLV
KEIDALGGAMAMATDKGGIQFRVLNSRKGPAVRATRAQADRVLYKAAVREILENQPNLWI
FQQAADDLIVEQEQVRGVVTQMGLRFFADSVVLTTGTFLGGLIHIGLQNFSGGRAGDPPS
IALAHRLRELPLRVGRLKTGTPPRIDGRSVDFSVMSEQPGDTPIPVMSFMGSKEQHPKQV
SCWITHTNARTHEIIAANLDRSPMYSAAGEIEGIGPRYCPSIEDKIHRFADKESHQVFIE
PEGLTTHELYPNGISTSLPFDVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLE
TKVIGGLFFAGQINGTTGYEEAGAQGLLAGTNAALRAQGKEAWCPRRDEAYIGVLVDDLI
TLGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDVRWAAFCKKRESIELEEQ
RLKSTWVRPGTEQGDAIAEKFGTPLTHEYNLLNLLSRPEIDYAGLIAVTGGGAEDPQVAE
QVEIKTKYAGYIDRQQDEIARLRASEDTKLPVDIDYTNISGLSKEIQSKLGATRPETLGQ
ASRIPGVTPAAISLLMIHLKKRGAGRQLEQSA