Protein Info for PfGW456L13_663 in Pseudomonas fluorescens GW456-L13

Annotation: DNA gyrase subunit B (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 TIGR01059: DNA gyrase, B subunit" amino acids 1 to 791 (791 residues), 985.6 bits, see alignment E=5.3e-301 PF02518: HATPase_c" amino acids 21 to 158 (138 residues), 60.7 bits, see alignment E=5.3e-20 PF00204: DNA_gyraseB" amino acids 209 to 378 (170 residues), 189.7 bits, see alignment E=9.2e-60 PF01751: Toprim" amino acids 407 to 519 (113 residues), 66.5 bits, see alignment E=6.1e-22 PF21249: GyrB_hook" amino acids 521 to 549 (29 residues), 61.2 bits, see alignment (E = 1.6e-20) PF18053: GyrB_insert" amino acids 552 to 717 (166 residues), 181.5 bits, see alignment E=3.9e-57 PF00986: DNA_gyraseB_C" amino acids 720 to 781 (62 residues), 105.9 bits, see alignment 2.8e-34

Best Hits

Swiss-Prot: 90% identical to GYRB_PSEPU: DNA gyrase subunit B (gyrB) from Pseudomonas putida

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 86% identity to avn:Avin_00040)

MetaCyc: 67% identical to DNA gyrase subunit B (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QSF1 at UniProt or InterPro

Protein Sequence (792 amino acids)

>PfGW456L13_663 DNA gyrase subunit B (EC 5.99.1.3) (Pseudomonas fluorescens GW456-L13)
VLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGYCDDISIIIHPDESITV
RDNGRGIPVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEELV
LTVRRSGKIWEQTYVHGVPQEPMKIVGDSETTGTQIHFKASSETFKNIHFSWDILAKRIR
ELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTPVNQVFHFNVQREDGIGV
EIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNTYIEAEGLAKKHKVATTG
DDAREGLTAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSDFLLENPNEAKLVV
GKMIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSA
KQGRNRKTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIGREEYNIDKLRYHN
IIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKKGKQEQYIKDDDAM
EEYMTQSALEDASLHLNEDAPGISGEALERLVNDFRLVMKTLKRLSRLYPQELTEHFIYL
PAVSMDQLSDHAAMQDWLAQYEVRLRTVEKSGLVYKASLREDRERGVWLPEVELISHGLS
NYVTFNRDFFGSNDYKTVVSLGAQLSTLLDDGAYIQRGERRKSVTEFKEALDWLMAESTK
RHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNTLMGDAVEPRRDFI
ESNALAVSNLDF