Protein Info for PfGW456L13_4770 in Pseudomonas fluorescens GW456-L13
Updated annotation (from data): ABC transporter for L-Asparagine and possibly other L-amino acids, periplasmic substrate-binding component
Rationale: Specific phenotype on L-Asparagine; Sodium octanoate. Detrimental on glutamine and might leak it. The mild phenotype on octanoate is not explained.
Original annotation: Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to GLTI_SALTY: Glutamate/aspartate import solute-binding protein (gltI) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K10001, glutamate/aspartate transport system substrate-binding protein (inferred from 96% identity to pba:PSEBR_a4408)MetaCyc: 57% identical to glutamate/aspartate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-13-RXN [EC: 7.4.2.1]; 7.4.2.1 [EC: 7.4.2.1]
Predicted SEED Role
"Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)" (TC 3.A.1.3.4)
Isozymes
No predicted isozymesUse Curated BLAST to search for 7.4.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0F4TN75 at UniProt or InterPro
Protein Sequence (304 amino acids)
>PfGW456L13_4770 ABC transporter for L-Asparagine and possibly other L-amino acids, periplasmic substrate-binding component (Pseudomonas fluorescens GW456-L13) MRIVPHILGAAIAAALISTPVFAAELTGTLKKIKESGTITLGHRDASIPFSYIADASGKP VGYSHDIQLKVVEALKKDLDMPNLQVKYNLVTSQTRIPLVQNGTVDLECGSTTNNVERQQ QVDFSVGIFEIGTRLLSKADSKYKDFPDLAGKNVVTTAGTTSERILKAMNADKQMGMNVI SAKDHGESFQMLETGRAVAFMMDDALLAGEAAKAKKASDWAVTGTPQSYEIYGCMVRKGD EPFKKAVDDAIKATYASGEINKIYEKWFMQPIPPKGLNLNFPMSDELKALIAKPTDKAAD DKKS