Protein Info for PfGW456L13_4240 in Pseudomonas fluorescens GW456-L13

Annotation: Adenosylcobinamide-phosphate synthase (EC 6.3.1.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03186: CobD_Cbib
  • amino acids 8 to 282 (275 residues), 325.3 bits, E=3.2e-101
• TIGR00380: cobalamin biosynthesis protein CobD
  • amino acids 8 to 269 (262 residues), 193.3 bits, E=3e-61
• PF17113: AmpE
  • amino acids 60 to 223 (164 residues), 35.5 bits, E=7.5e-13

Best Hits

Swiss-Prot: 73% identical to COBD_PSEU5: Cobalamin biosynthesis protein CobD (cobD) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K02227, adenosylcobinamide-phosphate synthase CobD [EC: 6.3.1.10] (inferred from 93% identity to pfo:Pfl01_1644)

Predicted SEED Role

"Adenosylcobinamide-phosphate synthase (EC 6.3.1.10)" (EC 6.3.1.10)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
• adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.10

Sequence Analysis Tools

See A0A293QSP0 at UniProt or InterPro

Protein Sequence (302 amino acids)

>PfGW456L13_4240 Adenosylcobinamide-phosphate synthase (EC 6.3.1.10) (Pseudomonas fluorescens GW456-L13)
MSVALLSVAAVALDALLGEPKRWHPLVAFGRFAERIEQRFNGGGRGWRSHGVTAWVIAVL
PLTLLATAFSWAPYVGWIVEILALYCALGMRSLGEHVTPVAKALRNNDLEAARTRVSYLV
SRQTSELDETEVARAATESVLENGSDAVFAALFWFAVAGAPGVVLYRLSNTLDAMWGYRN
ERFERFGWAAAKVDDVLNYIPARLVALTYALLGKTRLALKCWRTQAPIWDSPNAGPVMAA
GAGALGVELGGAAIYHGELHQRPQLGEGVPADADSIDRGWQLVQRGVWLWLLILCLGAEF
YA