Protein Info for PfGW456L13_3094 in Pseudomonas fluorescens GW456-L13

Annotation: formate dehydrogenase formation protein FdhE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 TIGR01562: formate dehydrogenase accessory protein FdhE" amino acids 3 to 305 (303 residues), 362.2 bits, see alignment E=1.4e-112 PF04216: FdhE" amino acids 16 to 303 (288 residues), 306.5 bits, see alignment E=1.5e-95

Best Hits

Swiss-Prot: 65% identical to FDHE_PSEPK: Protein FdhE homolog (fdhE) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02380, FdhE protein (inferred from 85% identity to pfo:Pfl01_2701)

Predicted SEED Role

"formate dehydrogenase formation protein FdhE" in subsystem Formate hydrogenase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QRF3 at UniProt or InterPro

Protein Sequence (308 amino acids)

>PfGW456L13_3094 formate dehydrogenase formation protein FdhE (Pseudomonas fluorescens GW456-L13)
LPTILEPGEIEAAASSPPFLYMPPHNLFSLRGQRLETLAEGHPMADYLRLIAGLCRVQQQ
VLDDPPPSDPLDRQRIELCQQHGLPPFAADSLAREDDWQPYLDALLQCYSAPSQSAVADA
VDTLRLVGAGQRRAWAVALVSGQYSLVPAALVPFLGAALQTAWSHWLLSTPNLALTPGDS
LSQCPACGSPAMAGVIRHRGKHNGLRYLVCSLCACEWHVVRVKCVYCEQSKGLEYLSLED
DRHAANQAPLRAEVCPGCNSYLKLLYLENDADAEALSADLSSLMLDMRLTQDGYQRLAPN
LLLAPGDE