Protein Info for PfGW456L13_2847 in Pseudomonas fluorescens GW456-L13
Annotation: 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 86% identical to METH_PSEAE: Methionine synthase (metH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 65% identity to abo:ABO_1324)Predicted SEED Role
"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)
MetaCyc Pathways
- aspartate superpathway (22/25 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- folate transformations III (E. coli) (9/9 steps found)
- folate transformations II (plants) (10/11 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
- folate transformations I (9/13 steps found)
- L-methionine biosynthesis I (3/5 steps found)
- L-methionine salvage from L-homocysteine (1/3 steps found)
- superpathway of L-methionine salvage and degradation (6/16 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.1.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293Q0V6 at UniProt or InterPro
Protein Sequence (1236 amino acids)
>PfGW456L13_2847 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) (Pseudomonas fluorescens GW456-L13) MSDRSVRLQALKHALKERILILDGGMGTMIQSYKLEEQDYRGKRFADWPSDVKGNNDLLV LTRPDVIGGIEKAYLDAGADILETNTFNATRISMADYGMEKLAYELNVEGARLARKVADA KTLENPDKPRFVAGVLGPTSRTCSLSPDVNNPGYRNVTFDELVENYTEATKGLIEGGADL ILIETIFDTLNAKAAIFAVQGVFEELGVELPIMISGTITDASGRTLSGQTTEAFWNSVAH AKPISVGLNCALGARELRPYLEELSNKANTHVSAHPNAGLPNEFGEYDELPSETAKVIEE FAQSGFLNIVGGCCGTTPGHIEAIAKAVAGYAPREIPDIPKACRLSGLEPFTIDRNSLFV NVGERTNITGSAKFARLIREDNYTEALEVALQQVEAGAQVIDINMDEGMLDSKKAMVTFL NLIAGEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISMKEGVEQFIHHAKLCKRYG AAVVVMAFDEAGQADTEARKKEICKRSYDILVNEVDFPPEDIIFDPNIFAVATGIEEHNN YAVDFINACAYIRDELPYALTSGGVSNVSFSFRGNNPVREAIHSVFLLYAIRSGLTMGIV NAGQLEIYDQIPVELRDAVEDVILNRTPEGTDALLAIADKYKGDGSVKEAETEEWRNWDV NKRLEHALVKGITTHIIEDTEESRQSFARPIEVIEGPLMAGMNIVGDLFGAGKMFLPQVV KSARVMKQAVAHLIPFIELEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIV DLGVMVPAEKILQVAREQKCDIIGLSGLITPSLDEMVHVAREMQRQDFHLPLMIGGATTS KAHTAVKIEPKYSNDAVIYVTDASRAVGVATQLLSKELKAGFVEKTRQDYIEVRERTSNR SARTERLSYGAAIAKKPQFDWASYTPVKPTFTGARVLDNIDLDVLAEYIDWTPFFISWDL AGKFPRILQDEVVGEAATSLYNDAREMLRKLIDEKLISARAVFGFWPANQVHDDDIELYG DDGKPMTKLHHLRQQIIKTDGKPNFSLADFVAPKDSEVTDYVGGFITTAGIGAEEVAKAY QDAGDDYNSIMVKALADRLAEACAEWLHQQVRKDYWGYAKDEALDNEALIKEQYSGIRPA PGYPACPDHTEKAQLFALLDPEAQEMRAGRSGVFLTEHYAMFPAAAVSGWYFAHPQAQYF AVGKIDKDQVQSYTSRKGQELSVTERWLAPNLGYDN