Protein Info for PfGW456L13_2178 in Pseudomonas fluorescens GW456-L13

Annotation: Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03807: F420_oxidored
  • amino acids 5 to 90 (86 residues), 29.1 bits, E=2.6e-10
• PF02153: PDH_N
  • amino acids 17 to 171 (155 residues), 130.1 bits, E=1.1e-41
• PF20463: PDH_C
  • amino acids 177 to 273 (97 residues), 96.2 bits, E=2.7e-31
• PF00275: EPSP_synthase
  • amino acids 306 to 719 (414 residues), 390 bits, E=2.4e-120
• TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase
  • amino acids 313 to 726 (414 residues), 422.1 bits, E=1.1e-130

Best Hits

KEGG orthology group: K00210, prephenate dehydrogenase [EC: 1.3.1.12] K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 98% identity to pfo:Pfl01_4074)

Predicted SEED Role

"Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43, EC 2.5.1.19)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• L-tyrosine biosynthesis I (3/3 steps found)
• L-tyrosine biosynthesis III (3/4 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• 4-hydroxy-3-prenylbenzoate biosynthesis (1/4 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Novobiocin biosynthesis
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.12 or 1.3.1.43 or 2.5.1.19

Sequence Analysis Tools

See A0A293QLJ7 at UniProt or InterPro

Protein Sequence (735 amino acids)

>PfGW456L13_2178 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) (Pseudomonas fluorescens GW456-L13)
MIGRLVVVGLGLIGGSFAKGLRESGLCREVVGVDLDPQSRKLAVELGVVDRCEEDLGVAC
QGADVIQLAVPILAMEKVLARLAGMDLGQAILTDVGSAKGNVVRAATEAFGKMPSCFVPG
HPIAGSEQSGVEASNAELFRRHKVILTPLEQTDPAALAVVDRLWRELGADVEHMQVERHD
EVLAATSHLPHLLAFGLVDSLAKRNENLEIFRYAAGGFRDFTRIAGSDPVMWHDIFLANR
EAVLRTLDTFRSDLDALRDAVDAGDGHQLLGVFTRARVAREHFSKILARRAYVDAMNSND
LIFLAQPGGRLSGRIRVPGDKSISHRSIMLGSLAEGVTEVEGFLEGEDALATLQAFRDMG
VVIEGPHHGRVTIHGVGLHGLKPAPGPIYLGNSGTSMRLLSGLLAAQNFDSTLTGDASLS
KRPMNRVANPLREMGAVIETAAEGRPPMTIRGGNKLKGLTYTMPMASAQVKSCLLLAGLY
AEGKTTVTEPAPTRDHTERMLRGFGYPVTVNGATASVESGNKLTATHIEVPGDISSSAFF
LVAASIAEGSELVLEHVGINPTRTGVIDILRLMGADITLENQREVGGEPVADLRVRAAKL
KGIEIPEALVPLAIDEFPVLFVAAACAEGRTVLRGAEELRVKESDRIQVMADGLLALGVR
CEPTPDGIIIDGGQIGGGEVHGHGDHRISMAFSVASLRASAPIRIHDCANVATSFPNFLA
LCAQVGIRVAQEAQS