Protein Info for PfGW456L13_1361 in Pseudomonas fluorescens GW456-L13

Annotation: Rod shape-determining protein RodA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 380 transmembrane" amino acids 26 to 48 (23 residues), see Phobius details amino acids 55 to 76 (22 residues), see Phobius details amino acids 83 to 104 (22 residues), see Phobius details amino acids 124 to 138 (15 residues), see Phobius details amino acids 148 to 165 (18 residues), see Phobius details amino acids 171 to 189 (19 residues), see Phobius details amino acids 195 to 213 (19 residues), see Phobius details amino acids 277 to 304 (28 residues), see Phobius details amino acids 316 to 340 (25 residues), see Phobius details amino acids 349 to 370 (22 residues), see Phobius details TIGR02210: rod shape-determining protein RodA" amino acids 27 to 375 (349 residues), 439.3 bits, see alignment E=5.5e-136 PF01098: FTSW_RODA_SPOVE" amino acids 38 to 375 (338 residues), 371 bits, see alignment E=3e-115

Best Hits

Swiss-Prot: 53% identical to RODA_ECOLI: Peptidoglycan glycosyltransferase MrdB (mrdB) from Escherichia coli (strain K12)

KEGG orthology group: K05837, rod shape determining protein RodA (inferred from 96% identity to pfo:Pfl01_4969)

MetaCyc: 53% identical to peptidoglycan glycosyltransferase MrdB (Escherichia coli K-12 substr. MG1655)
Peptidoglycan glycosyltransferase. [EC: 2.4.1.129]

Predicted SEED Role

"Rod shape-determining protein RodA" in subsystem Bacterial Cytoskeleton or Peptidoglycan Biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129

Use Curated BLAST to search for 2.4.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293Q7H0 at UniProt or InterPro

Protein Sequence (380 amino acids)

>PfGW456L13_1361 Rod shape-determining protein RodA (Pseudomonas fluorescens GW456-L13)
MNNFDRMLSSEDVMRRRATLLQRLHIDGPLLILLLTLAAGSLFVLYSASGKSWDLLAKQA
TSFGIGLVSMIVIAQFEPRFMARWVPVGYVIGVLLLVVVDVMGHNAMGATRWINIPGVIR
FQPSEFMKILMPATIAWYLSKRTLPPQLKHVGVSLFLIGLPFILIVRQPDLGTSLLILAG
GAFVLFMGGLRWRWILSVLAAAVPVSVAMWYFVMHDYQKQRVLTFLDPESDPLGTGWNII
QSKAAIGSGGVFGKGWLLGTQSHLDFLPESHTDFIIAVLGEEFGLVGICALLLIYLLLIG
RGLVITAQAQTLFGKLLAGSLTMTFFVYVFVNIGMVSGLLPVVGVPLPFISYGGTSLVTL
LSAFGVLMSIHTHRKWIAQV