Protein Info for PfGW456L13_1092 in Pseudomonas fluorescens GW456-L13

Annotation: Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 PF06155: GBBH-like_N" amino acids 27 to 104 (78 residues), 45.9 bits, see alignment E=7.8e-16 TIGR02409: gamma-butyrobetaine hydroxylase" amino acids 29 to 387 (359 residues), 449.3 bits, see alignment E=4.9e-139 PF02668: TauD" amino acids 136 to 372 (237 residues), 158.3 bits, see alignment E=3.7e-50

Best Hits

Swiss-Prot: 69% identical to BODG_PSESK: Gamma-butyrobetaine dioxygenase from Pseudomonas sp. (strain AK-1)

KEGG orthology group: K00471, gamma-butyrobetaine dioxygenase [EC: 1.14.11.1] (inferred from 87% identity to pfo:Pfl01_5234)

MetaCyc: 69% identical to gamma-butyrobetaine hydroxylase subunit (Pseudomonas sp. AK-1)
Gamma-butyrobetaine dioxygenase. [EC: 1.14.11.1]

Predicted SEED Role

"Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1)" (EC 1.14.11.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QKR0 at UniProt or InterPro

Protein Sequence (387 amino acids)

>PfGW456L13_1092 Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1) (Pseudomonas fluorescens GW456-L13)
MNTAAAFADFRTYPLISALTAVQTLADRVLVKWADNRVSPFHHQWLRDNCPCPQCVYTVT
REQVLEIVDTAQDLIPDQVHIDTEGCLSVDWQDGHSSRFDPGWLRAHAYDDESRAERQAR
KPKARLWHSNLQLPVFEYQALMNDSDALLQWLLAVRDVGLTQVRGVLTEPGSLKLIAQRI
SFIRESNFGVLFNVQSKADADSNAYTAFNLPLHSDLPTRELQPGLQFLHCLVNDAEGGES
IFVDGFAIADALRREDPESFKSLCEIPVEFRNKDRHSDYRCLAPIIALDTLGQVSEIRMA
NFLRGPFDASVEQMPKLYQAYRRFIAMTREPRFRLMQRLNPGELWCFDNRRTLHARNAFD
PATGARHFQGCYVDRDELLSRILVLQR